[Histonet] destaining cyto slides for immuno

[Tony Henwood]

Jen,

I would assume that the cyto slide was either air-dried or ethanol
fixed.
You do not need to heat retrieve nor enzyme treat.
Just remove the coverslip, (give a good soak in xylene or equivalent to
remove all the mountant), re-hydrate to water, block endogenous
peroxidase, add the AE1/AE3 antibody and proceed as usual.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer
Campbell
Sent: Saturday, 15 August 2009 12:13 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] destaining cyto slides for immuno


Hi All,
 
  How would you go about destaining a cyto slide and running a
CKAE1/AE3?  I was told that acid alcohol to remove the stain beforehand
may not be necessary as heat retrieval may remove it adequately enough.
Does anyone have any recommendations?
 
Thanks,
 
Jen
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