[Histonet] destaining cyto slides for immuno

[Sebree Linda A]

We used to destain but after a while decided that whatever remaining PAP
stain would not interfere with interpretation...most of it is gone after
IHC anyway.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer
Campbell
Sent: Friday, August 14, 2009 9:13 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] destaining cyto slides for immuno


Hi All,
 
  How would you go about destaining a cyto slide and running a
CKAE1/AE3?  I was told that acid alcohol to remove the stain beforehand
may not be necessary as heat retrieval may remove it adequately enough.
Does anyone have any recommendations?
 
Thanks,
 
Jen
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