[Histonet] optimal IHC thickness
Rene J Buesa
rjbuesa <@t> yahoo.com
Thu Aug 13 10:23:51 CDT 2009
I always used 3-4µm and prefer HIER over enzymes.
--- On Thu, 8/13/09, Katelin Lester <katelin <@t> cuttingedgehistology.com> wrote:
From: Katelin Lester <katelin <@t> cuttingedgehistology.com>
Subject: [Histonet] optimal IHC thickness
To: histonet <@t> lists.utsouthwestern.edu
Date: Thursday, August 13, 2009, 10:24 AM
I am staining lymph nodes for staging of breast cancer with CKAE1/AE3 and
would like to know what everyone is cutting this type of slide at.
I searched the archives and my understanding is that, for IHC, how thick the
sections are cut is not necessarily critical. I typically cut all IHC at
4μm and I want to achieve the best results possible.
Also, on a side note, the Data Sheet suggests using Trypsin, but I have been
using Pepsin. Any comments or suggestions about time, quality of Trypsin vs.
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