[Histonet] advice about bone section please

Wei-Hsiang Huang weihsianghuang <@t> gmail.com
Thu Aug 13 13:27:54 CDT 2009

Hi all, I'm planning to do paraffin embed section on mouse joint (E12~P10)
to detect the GFP-tagged protein expression. Since bone has
autofluorescence, I'm wondering whether IHC would be a good choice for
postnatal bone, and can I do immunofluorescence on joint in E12~16 mouse
without having autofluorescence effect?

I've tried cryosection (10 micrometer) but the bone detached and doesn't
give me good morphology so I decided to try paraffin.

thank you all in advance.


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