[Histonet] Fixation of embryos

[Geoff McAuliffe]

If the G in 4F1G is glutaraldehyde the glut. will probably kill off the 
immunoreactivity (depending on the antigen in question).
How long are the embryos being fixed? Remember, formalin works slowly so 
5-7 days may be necessary.-
I would try a formalin + alcohol mix or formalin-alcohol-acetic acid.
Also, yolk is hard to fix well so don't use that as a criterion for good 
fixation.

Geoff

Cheryl Crowder wrote:
> Hello - A researcher is fixing 7-21 day old chicken embryos in 10% NBF.  
> After processing they are mushy and almost amorphous.  Has anyone any 
> suggestions for a fixative that will give the embryos more form and still 
> have the tissue capable of doing IHC?   I had thought of 4F1G.  Any help is 
> really appreciated.
> Cheryl
>  
> Cheryl Crowder, BA, HTL(ASCP)
> Chief Technologist
> Anatomic Pathology
> Department of Pathobiological Sciences
> School of Veterinary Medicine
> Louisiana State University
> Skip Bertman Drive
> Baton Rouge, LA 70803
>
> 225-578-9734
> FAX: 225-578-9720
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
**********************************************