[Histonet] Fixation of embryos
Cheryl Crowder
ccrowder <@t> vetmed.lsu.edu
Mon Aug 10 10:27:21 CDT 2009
Hello - A researcher is fixing 7-21 day old chicken embryos in 10% NBF.
After processing they are mushy and almost amorphous. Has anyone any
suggestions for a fixative that will give the embryos more form and still
have the tissue capable of doing IHC? I had thought of 4F1G. Any help is
really appreciated.
Cheryl
Cheryl Crowder, BA, HTL(ASCP)
Chief Technologist
Anatomic Pathology
Department of Pathobiological Sciences
School of Veterinary Medicine
Louisiana State University
Skip Bertman Drive
Baton Rouge, LA 70803
225-578-9734
FAX: 225-578-9720
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