[Histonet] Fixation of embryos

Rene J Buesa rjbuesa <@t> yahoo.com
Mon Aug 10 11:54:45 CDT 2009

Use Bouin's fixative.
René J.

--- On Mon, 8/10/09, Cheryl Crowder <ccrowder <@t> vetmed.lsu.edu> wrote:

From: Cheryl Crowder <ccrowder <@t> vetmed.lsu.edu>
Subject: [Histonet] Fixation of embryos
To: "Histonet" <histonet <@t> pathology.swmed.edu>
Date: Monday, August 10, 2009, 11:27 AM

Hello - A researcher is fixing 7-21 day old chicken embryos in 10% NBF.  
After processing they are mushy and almost amorphous.  Has anyone any 
suggestions for a fixative that will give the embryos more form and still 
have the tissue capable of doing IHC?   I had thought of 4F1G.  Any help is 
really appreciated.

Cheryl Crowder, BA, HTL(ASCP)
Chief Technologist
Anatomic Pathology
Department of Pathobiological Sciences
School of Veterinary Medicine
Louisiana State University
Skip Bertman Drive
Baton Rouge, LA 70803

FAX: 225-578-9720
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu


More information about the Histonet mailing list