[Histonet] Clear-Rite 3

[Sherwood, Margaret]

We also use d-limonene (sold as CitriSolv).  We used Hemo-De before.  I also
would be curious you (and others) do not like d-limonene.

Peggy 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Va Paula
Sicurello
Sent: Wednesday, August 05, 2009 12:49 PM
To: Rene J Buesa; HistoNet
Subject: Re: [Histonet] Clear-Rite 3

Hi Rene,

Why do you not like the d-limonene solvents? 

I work with them and the results seem fine.  I have to mention that I use an
AutoTechnicon Duo and cannot place it in a fume hood to vent the xylene vapors. 
Without proper ventilation the safety people willnot allow the use of Xylenen. 
I work in the research unit of a VA hospital and they will not provide larger
fume hoods nor buy me a self contained processing sytem.

Please let me know what other safe alternatives (ones that can be used out on
the bench) are you suggest.

Thanks,

Paula Sicurello

VA Medical Center San Diego

Veterans Medical Research Foundation (VMRF) 

Core for Micro Imaging(C-MI)

3350 La Jolla Village Dr., MC151

San Diego, CA 92161

858-552-8585 x2397



C-MI for your imaging needs.

--- On Wed, 8/5/09, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Clear-Rite 3
To: Histonet <@t> lists.utsouthwestern.edu, "BillO'Donnell"
<billodonnell <@t> catholichealth.net>
Date: Wednesday, August 5, 2009, 2:01 PM

Bill:
Xylene substitutes are not all "pretty much the same". There are d-Limonene
derivatives (something to stay absolutely away from) and alkane derivatives with
many known and unknown constituents.
Since you are about to change and not have any preference, your best option both
in processing quality and price, would be to get a reliable supplier of "mineral
spirits" (the same you can find at any home improvement store) and use it.
René J. 

--- On Wed, 8/5/09, O'Donnell, Bill <billodonnell <@t> catholichealth.net> wrote:


From: O'Donnell, Bill <billodonnell <@t> catholichealth.net>
Subject: [Histonet] Clear-Rite 3
To: Histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, August 5, 2009, 9:11 AM


Greetings!

We have been using Clear-Rite 3 here at our lab, and we are happy with
the product. Our supplier says it will be on back-order for some time
now. Our crack supply folks are looking for another source. I'm taking
another route to find out what products out there are comparable.

Are all "Xylene Substitutes" pretty much the same and there for pretty
much interchangable?
Are there some to stay away from?

Any help is appriciated.

William (Bill) O'Donnell, HT (ASCP) QIHC 
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 
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