[Histonet] Clear-Rite 3

[Victor Tobias]

The last time I was around a citrus based clearing agent was in the 80's 
and it gave me severe headaches. I didn't have any problems with it's 
performance, just the health aspects. I was using a closed processor at 
the time, but no hood..

Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor <@t> pathology.washington.edu
206-598-2792
206-598-7659 Fax
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Sherwood, Margaret wrote:
> We also use d-limonene (sold as CitriSolv).  We used Hemo-De before.  I also
> would be curious you (and others) do not like d-limonene.
>
> Peggy 
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Va Paula
> Sicurello
> Sent: Wednesday, August 05, 2009 12:49 PM
> To: Rene J Buesa; HistoNet
> Subject: Re: [Histonet] Clear-Rite 3
>
> Hi Rene,
>
> Why do you not like the d-limonene solvents? 
>
> I work with them and the results seem fine.  I have to mention that I use an
> AutoTechnicon Duo and cannot place it in a fume hood to vent the xylene vapors. 
> Without proper ventilation the safety people willnot allow the use of Xylenen. 
> I work in the research unit of a VA hospital and they will not provide larger
> fume hoods nor buy me a self contained processing sytem.
>
> Please let me know what other safe alternatives (ones that can be used out on
> the bench) are you suggest.
>
> Thanks,
>
> Paula Sicurello
>
> VA Medical Center San Diego
>
> Veterans Medical Research Foundation (VMRF) 
>
> Core for Micro Imaging(C-MI)
>
> 3350 La Jolla Village Dr., MC151
>
> San Diego, CA 92161
>
> 858-552-8585 x2397
>
>
>
> C-MI for your imaging needs.
>
> --- On Wed, 8/5/09, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
>
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Clear-Rite 3
> To: Histonet <@t> lists.utsouthwestern.edu, "BillO'Donnell"
> <billodonnell <@t> catholichealth.net>
> Date: Wednesday, August 5, 2009, 2:01 PM
>
> Bill:
> Xylene substitutes are not all "pretty much the same". There are d-Limonene
> derivatives (something to stay absolutely away from) and alkane derivatives with
> many known and unknown constituents.
> Since you are about to change and not have any preference, your best option both
> in processing quality and price, would be to get a reliable supplier of "mineral
> spirits" (the same you can find at any home improvement store) and use it.
> René J. 
>
> --- On Wed, 8/5/09, O'Donnell, Bill <billodonnell <@t> catholichealth.net> wrote:
>
>
> From: O'Donnell, Bill <billodonnell <@t> catholichealth.net>
> Subject: [Histonet] Clear-Rite 3
> To: Histonet <@t> lists.utsouthwestern.edu
> Date: Wednesday, August 5, 2009, 9:11 AM
>
>
> Greetings!
>
> We have been using Clear-Rite 3 here at our lab, and we are happy with
> the product. Our supplier says it will be on back-order for some time
> now. Our crack supply folks are looking for another source. I'm taking
> another route to find out what products out there are comparable.
>
> Are all "Xylene Substitutes" pretty much the same and there for pretty
> much interchangable?
> Are there some to stay away from?
>
> Any help is appriciated.
>
> William (Bill) O'Donnell, HT (ASCP) QIHC 
> Lead Histologist
> Good Samaritan Hospital
> 10 East 31st Street
> Kearney, NE 68847 
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