[Histonet] Clear-Rite 3

Va Paula Sicurello vapatpxs <@t> yahoo.com
Wed Aug 5 11:48:59 CDT 2009

Hi Rene,

Why do you not like the d-limonene solvents? 

I work with them and the results seem fine.  I have to mention that I use an AutoTechnicon Duo and cannot place it in a fume hood to vent the xylene vapors.  Without proper ventilation the safety people willnot allow the use of Xylenen.  I work in the research unit of a VA hospital and they will not provide larger fume hoods nor buy me a self contained processing sytem.

Please let me know what other safe alternatives (ones that can be used out on the bench) are you suggest.


Paula Sicurello

VA Medical Center San Diego

Veterans Medical Research Foundation (VMRF) 

Core for Micro Imaging(C-MI)

3350 La Jolla Village Dr., MC151

San Diego, CA 92161

858-552-8585 x2397

C-MI for your imaging needs.

--- On Wed, 8/5/09, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Clear-Rite 3
To: Histonet <@t> lists.utsouthwestern.edu, "BillO'Donnell" <billodonnell <@t> catholichealth.net>
Date: Wednesday, August 5, 2009, 2:01 PM

Xylene substitutes are not all "pretty much the same". There are d-Limonene derivatives (something to stay absolutely away from) and alkane derivatives with many known and unknown constituents.
Since you are about to change and not have any preference, your best option both in processing quality and price, would be to get a reliable supplier of "mineral spirits" (the same you can find at any home improvement store) and use it.
René J. 

--- On Wed, 8/5/09, O'Donnell, Bill <billodonnell <@t> catholichealth.net> wrote:

From: O'Donnell, Bill <billodonnell <@t> catholichealth.net>
Subject: [Histonet] Clear-Rite 3
To: Histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, August 5, 2009, 9:11 AM


We have been using Clear-Rite 3 here at our lab, and we are happy with
the product. Our supplier says it will be on back-order for some time
now. Our crack supply folks are looking for another source. I'm taking
another route to find out what products out there are comparable.

Are all "Xylene Substitutes" pretty much the same and there for pretty
much interchangable?
Are there some to stay away from?

Any help is appriciated.

William (Bill) O'Donnell, HT (ASCP) QIHC 
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 
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