[Histonet] Vectashield mounting media problem?

[Montina Van Meter]

ProLong Gold w/ DAPI  (Invitrogen/Molecular Probes)

This is a permanent mounting media - works great!




Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791
225-763-2564

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Suhyoung
Jeong
Sent: Tuesday, April 21, 2009 9:52 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Vectashield mounting media problem?

Hello everyone,

Our lab has been using 'Vectashield mounting media w/ DAPI' for many
years
and had no problem of fading. Recently, two bottles with different lot
numbers in a row gone bad in around four months (the signal disappears
in
front of your eyes, tested on a couple of different microscopes).

1) Has anyone experienced same problem? Vector lab only says that this
is a
very stable product and should not be happening when stored at 4C.

2) Can anyone recommend a good aqueous mounting media with DAPI for
immunofluorescence?

Thank you in advance,

Regards, Suh
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