[Histonet] Vectashield mounting media problem?

[Suhyoung Jeong]

Hello everyone,

Our lab has been using 'Vectashield mounting media w/ DAPI' for many years
and had no problem of fading. Recently, two bottles with different lot
numbers in a row gone bad in around four months (the signal disappears in
front of your eyes, tested on a couple of different microscopes).

1) Has anyone experienced same problem? Vector lab only says that this is a
very stable product and should not be happening when stored at 4C.

2) Can anyone recommend a good aqueous mounting media with DAPI for
immunofluorescence?

Thank you in advance,

Regards, Suh