[Histonet] CNS Fixation

[Colleen Forster]

Animal brain is the same. IF you aior dry overnight and then into the 
oven before starting your stains you will not lose sections.

Colleen Forster
U of MN


Barbara Albert wrote:
> We've found this also.  Even 30-60 minutes air drying helps alot with human brain. We use regular slides and NBF.
>
> Barbara Albert
> UCSF Medical Center
> San Francisco
>
>
>
>
> ________________________________
> From: "Weems, Joyce" <JWeems <@t> sjha.org>
> To: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>; histonet <@t> lists.utsouthwestern.edu
> Sent: Friday, April 17, 2009 7:49:49 AM
> Subject: RE: [Histonet] CNS Fixation
>
> I have found that brain sections that are air-dried overnight - then
> dried in the oven rarely ever wash off. We fix in 10% NBF. This is human
> - not sure if animal would be different. 
>
> Best, j
>
>
> Joyce Weems 
> Pathology Manager 
> Saint Joseph's Hospital 
> 5665 Peachtree Dunwoody Rd NE 
> Atlanta, GA 30342 
> 678-843-7376 - Phone 
> 678-843-7831 - Fax 
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Breeden,
> Sara
> Sent: Friday, April 17, 2009 10:37 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] CNS Fixation
>
> Yes, I'm back.  I need the expertise of this group.
>
>
>
> I am trying to convince my three pathologists that fixation in alcoholic
> formalin is the best route for whole brain and spinal cord.  This has
> been an uphill battle and in order to prevent sections from peeling off
> during staining, I am still pre-drying necropsy brain slides for an
> extra 20 minutes before putting on the auto-stainer (even this does not
> prevent all instances). I need some expert ammunition for my "battle"
> despite the fact that the SOP I've written requires it and it is rarely
> followed.  If alcoholic formalin is not as good as some other method,
> I'm open to suggestion. Of course, convincing them that thinner sections
> would also help - but one step at a time!  Thank you in advance!
>
>
>
> Sally Breeden, HT(ASCP)
>
> NM Dept. of Agriculture
>
> Veterinary Diagnostic Services
>
> PO Box 4700
>
> Albuquerque, NM  87106
>
> 505-841-2576
>
>
>
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