[Histonet] CNS Fixation

[Barbara Albert]

We've found this also.  Even 30-60 minutes air drying helps alot with human brain. We use regular slides and NBF.

Barbara Albert
UCSF Medical Center
San Francisco




________________________________
From: "Weems, Joyce" <JWeems <@t> sjha.org>
To: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>; histonet <@t> lists.utsouthwestern.edu
Sent: Friday, April 17, 2009 7:49:49 AM
Subject: RE: [Histonet] CNS Fixation

I have found that brain sections that are air-dried overnight - then
dried in the oven rarely ever wash off. We fix in 10% NBF. This is human
- not sure if animal would be different. 

Best, j


Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Friday, April 17, 2009 10:37 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CNS Fixation

Yes, I'm back.  I need the expertise of this group.



I am trying to convince my three pathologists that fixation in alcoholic
formalin is the best route for whole brain and spinal cord.  This has
been an uphill battle and in order to prevent sections from peeling off
during staining, I am still pre-drying necropsy brain slides for an
extra 20 minutes before putting on the auto-stainer (even this does not
prevent all instances). I need some expert ammunition for my "battle"
despite the fact that the SOP I've written requires it and it is rarely
followed.  If alcoholic formalin is not as good as some other method,
I'm open to suggestion. Of course, convincing them that thinner sections
would also help - but one step at a time!  Thank you in advance!



Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106

505-841-2576



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