[Histonet] CNS Fixation

[Geoff McAuliffe]

Hi Sara:

I, and it seems others, disagree with the idea that alcoholic formalin 
is the best fixative for CNS. Buffered formalin is the fix of choice for 
animal and human CNS.

Geoff

Breeden, Sara wrote:
> Yes, I'm back.  I need the expertise of this group.
>
>  
>
> I am trying to convince my three pathologists that fixation in alcoholic
> formalin is the best route for whole brain and spinal cord.  This has
> been an uphill battle and in order to prevent sections from peeling off
> during staining, I am still pre-drying necropsy brain slides for an
> extra 20 minutes before putting on the auto-stainer (even this does not
> prevent all instances). I need some expert ammunition for my "battle"
> despite the fact that the SOP I've written requires it and it is rarely
> followed.  If alcoholic formalin is not as good as some other method,
> I'm open to suggestion. Of course, convincing them that thinner sections
> would also help - but one step at a time!  Thank you in advance!
>
>  
>
> Sally Breeden, HT(ASCP)
>
> NM Dept. of Agriculture
>
> Veterinary Diagnostic Services
>
> PO Box 4700
>
> Albuquerque, NM  87106
>
> 505-841-2576
>
>  
>
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>
>
>   


-- 
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
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