[Histonet] RE: TMAs for Cell Pellets - Histonet Digest, Vol 65,
Issue 22
Thom Jensen
tissuearray <@t> hotmail.com
Wed Apr 15 22:41:43 CDT 2009
If you are making a cell blocks with your cell pellets then punching the cell block and inserting it into a TMA then is ideal. The cell pellets we have made were created like a cell block. The cells are spon down, the fluid is poured off and the cell button is mixed with a augar type substance (venders would have info on the augar type material used for making cell blocks). Once the solution solidifies it is placed in a paper or mesh bage and placed into a cassette for processing. Once processed the cells are imbedded into a paraffin block as you would tissue. A fresh H&E is cut off the block and areas of interest are marked on the slide to show the best spot to punch for the TMA. You will also find that the TMA instrument can pay for itself sometimes after one protocal. By this I mean you will save on antibody cost and have consistency in your staining since all samples are on one slide instead of many slides. I have constructed regular paraffin TMAs, cell arrays, sponge arrays (cells growing in a sponge type material processed and embedded in paraffin) and frozen TMAs. It just takes a little expermenting, some patience and pratice making TMAs.
cheers,
Thom
> Date: Tue, 14 Apr 2009 10:20:40 -0400
> From: lac65 <@t> email.med.yale.edu
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: Histonet Digest, Vol 65, Issue 22
>
> Give me a shout in regards to cell pellets & TMA's.
> There are several methods and some are less expensive then others.
> Have a good one.
> Lori
>
>
>
> Beecher is usually difficult to deal
> with.histonet-request <@t> lists.utsouthwestern.edu wrote:
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> > Today's Topics:
> >
> > 1. Re: TMA for cell pellets? (Bernie Taupin)
> > 2. To all spam gourmets (V. Neubert)
> > 3. RE: I recommend NOT opening that Attachment: (Hugh Luk)
> > 4. RE: TMA for cell pellets? (Hartson, Louise)
> > 5. Re: Formula 83 users? (Rene J Buesa)
> > 6. Re: Negative IHC controls (Rene J Buesa)
> > 7. RE: Formula 83 users? (Blazek, Linda)
> > 8. Re: Spyware from an Attachment: (Geoff McAuliffe)
> > 9. Formula 83-Thank you! (Jacqueline Farnsworth)
> > 10. HISTOS 5 processor (FU,DONGTAO)
> > 11. Position Open In Mass (Alyssa Peterson)
> > 12. Re: HISTOS 5 processor (Robert Schoonhoven)
> > 13. Travelling Histotechs (Robert Schoonhoven)
> > 14. Regional Sales Manager position- West Coast
> > (Kris.Caldwell <@t> leica-microsystems.com)
> > 15. New lab, need some info (Putnam, Jodi)
> >
> >
> > ----------------------------------------------------------------------
> >
> > Message: 1
> > Date: Wed, 8 Apr 2009 22:50:26 -0700 (PDT)
> > From: Bernie Taupin <bernietaupin <@t> ymail.com>
> > Subject: Re: [Histonet] TMA for cell pellets?
> > To: Thom Jensen <tissuearray <@t> hotmail.com>,
> > louise_hartson <@t> urmc.rochester.edu, histonet <@t> lists.utsouthwestern.edu
> > Message-ID: <338565.20208.qm <@t> web43513.mail.sp1.yahoo.com>
> > Content-Type: text/plain; charset=iso-8859-1
> >
> > Recently, someone mentioned putting a library of protocols online, perhaps in the HistoNet archives. I'd like to second that emotion if its possible to make happen... What a great resource that would be!
> >
> >
> >
> >
> > ________________________________
> > From: Thom Jensen <tissuearray <@t> hotmail.com>
> > To: louise_hartson <@t> urmc.rochester.edu; histonet <@t> lists.utsouthwestern.edu
> > Sent: Thursday, April 9, 2009 1:27:18 AM
> > Subject: [Histonet] TMA for cell pellets?
> >
> >
> >
> > Have you tried to embed your cell pellets into a tissue microarray? Two companies I would recommend:
> > www.arraymold.com
> > www.beecherinstruments.com
> >
> > Two of the best TMA products on the market.
> >
> >
> > cheers,
> >
> >
> >> Date: Thu, 2 Apr 2009 12:05:39 -0400
> >> From: Louise_Hartson <@t> URMC.Rochester.edu
> >> To: histonet <@t> lists.utsouthwestern.edu
> >> Subject: [Histonet] Embedding cell pellets
> >>
> >>
> >> I am looking for a protocol for embedding cell pellets in paraffin.
> >> Thanks,
> >> Louise
> >>
> >> Louise Hartson, BA
> >> Senior Technical Associate
> >> University of Rochester
> >> Louise_Hartson <@t> URMC.Rochester.edu
> >> _______________________________________________
> >> Histonet mailing list
> >> Histonet <@t> lists.utsouthwestern.edu
> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >>
> >
> > _________________________________________________________________
> > Hotmail® is up to 70% faster. Now good news travels really fast.
> > http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009_______________________________________________
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> >
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 2
> > Date: Thu, 09 Apr 2009 10:07:54 +0200
> > From: "V. Neubert" <histonet.nospam <@t> vneubert.com>
> > Subject: [Histonet] To all spam gourmets
> > To: histonet <@t> lists.utsouthwestern.edu
> > Message-ID: <49DDACDA.8040503 <@t> vneubert.com>
> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed
> >
> > Please, stop spamming this list.
> > Comment if you know something useful. Otherwise keep it.
> > I don't mind what you had for breakfast in June 1st last year or your
> > opinion on my clothes' colour.
> >
> > Believe it or not, there IS a real life ( link:
> > http://en.wikipedia.org/wiki/Real_life_(reality) ), and maybe you should
> > check it out - it has way more to offer than waiting for responses to
> > mock about.
> >
> > Thanks.
> > Valentin
> >
> > PS: What about a moderated board? Every inappropriate comment on HISTO
> > TOPIC could be moved or even deleted, except in the spamming forum which
> > can be found on nearly every board on the net.
> >
> >
> >
> > ------------------------------
> >
> > Message: 3
> > Date: Wed, 8 Apr 2009 22:13:22 -1000
> > From: Hugh Luk <hlukey <@t> msn.com>
> > Subject: RE: [Histonet] I recommend NOT opening that Attachment:
> > To: histonet <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID: <SNT106-W30443621932D763B828123C3830 <@t> phx.gbl>
> > Content-Type: text/plain; charset="iso-8859-1"
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> > Fellow histology and related fields professionals,
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> >
> > I'm not opening this. From a call name of "u know (u_deserve_2_know <@t> yahoo.com)." The last time I opened something like this, from a name like this, I ended up owing money for Viagra treatments for Panda bears or something like that. It may be real, but it probably is spam. There...has been...a lot...of it...recently!
> >
> > By the way, there is no such thing as a "Virus-proof LINK!" Not sure? Just Google it.
> >
> > If you need Spyware or Antivirus software, check your IT department. Also, CNET has free downloads of AdAware, or AVG antivirus 8.
> >
> > And now for a joke (I think we all need it); A physician is doing his rounds and a nurse stops him in the hallway, "Doctor, can you sign these reports?" "Fine" he says and proceeds to pull out a rectal thermometer from his coat pocket. He looks at it in disbelief and exclaims, "Darn it! Some a$$ has my pen!"
> >
> > Respectfully,
> > Hugh Luk, HTL (ASCP)
> > Cancer Research center of Hawaii
> > Pathology shared resources lab manager
> > KP- Hawaii
> >
> >
> >
> >
> >> Date: Wed, 8 Apr 2009 21:41:35 -0700
> >> From: u_deserve_2_know <@t> yahoo.com
> >> To: histonet <@t> lists.utsouthwestern.edu
> >> Subject: [Histonet] Spyware from an Attachment:
> >>
> >> Dear Listers,
> >>
> >> If you ever opened any strange attachments from this list, you should really consider scanning your Hard Disk for spyware. There is a great free anti-spyware program we use, which you can get at http://DELETED<----that is a virus-proof link, by the way.
> >>
> >>
> >>
> >>
> >> _______________________________________________
> >> Histonet mailing list
> >> Histonet <@t> lists.utsouthwestern.edu
> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >>
> >
> > _________________________________________________________________
> > Quick access to your favorite MSN content and Windows Live with Internet Explorer 8.
> > http://ie8.msn.com/microsoft/internet-explorer-8/en-us/ie8.aspx?ocid=B037MSN55C0701A
> >
> > ------------------------------
> >
> > Message: 4
> > Date: Thu, 9 Apr 2009 08:29:23 -0400
> > From: "Hartson, Louise" <Louise_Hartson <@t> URMC.Rochester.edu>
> > Subject: RE: [Histonet] TMA for cell pellets?
> > To: "Bernie Taupin" <bernietaupin <@t> ymail.com>, "Thom Jensen"
> > <tissuearray <@t> hotmail.com>, <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> > <1089A756B010074CA09E65CD92401608018A8CAC <@t> MEDMAIL.urmc-sh.rochester.edu>
> >
> > Content-Type: text/plain; charset="iso-8859-1"
> >
> > Thank you to everyone who responded!! I was given the cell pellets already in the matrix so I only had to process!! It was easier than the mouse tissues!! Since we do so few 20-40 at a time I do everything by hand...no automation in our lab!!
> >
> >
> > -----Original Message-----
> > From: Bernie Taupin [mailto:bernietaupin <@t> ymail.com]
> > Sent: Thu 4/9/2009 1:50 AM
> > To: Thom Jensen; Hartson, Louise; histonet <@t> lists.utsouthwestern.edu
> > Subject: Re: [Histonet] TMA for cell pellets?
> >
> > Recently, someone mentioned putting a library of protocols online, perhaps in the HistoNet archives. I'd like to second that emotion if its possible to make happen... What a great resource that would be!
> >
> >
> >
> >
> > ________________________________
> > From: Thom Jensen <tissuearray <@t> hotmail.com>
> > To: louise_hartson <@t> urmc.rochester.edu; histonet <@t> lists.utsouthwestern.edu
> > Sent: Thursday, April 9, 2009 1:27:18 AM
> > Subject: [Histonet] TMA for cell pellets?
> >
> >
> >
> > Have you tried to embed your cell pellets into a tissue microarray? Two companies I would recommend:
> > www.arraymold.com
> > www.beecherinstruments.com
> >
> > Two of the best TMA products on the market.
> >
> >
> > cheers,
> >
> >
> >> Date: Thu, 2 Apr 2009 12:05:39 -0400
> >> From: Louise_Hartson <@t> URMC.Rochester.edu
> >> To: histonet <@t> lists.utsouthwestern.edu
> >> Subject: [Histonet] Embedding cell pellets
> >>
> >>
> >> I am looking for a protocol for embedding cell pellets in paraffin.
> >> Thanks,
> >> Louise
> >>
> >> Louise Hartson, BA
> >> Senior Technical Associate
> >> University of Rochester
> >> Louise_Hartson <@t> URMC.Rochester.edu
> >> _______________________________________________
> >> Histonet mailing list
> >> Histonet <@t> lists.utsouthwestern.edu
> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >>
> >
> > _________________________________________________________________
> > Hotmail® is up to 70% faster. Now good news travels really fast.
> > http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009_______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 5
> > Date: Thu, 9 Apr 2009 06:56:21 -0700 (PDT)
> > From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> > Subject: Re: [Histonet] Formula 83 users?
> > To: Histonet <Histonet <@t> lists.utsouthwestern.edu>, Jacqueline
> > Farnsworth <Jacqueline.Farnsworth <@t> cls.ab.ca>
> > Message-ID: <613905.80912.qm <@t> web65715.mail.ac4.yahoo.com>
> > Content-Type: text/plain; charset=iso-8859-1
> >
> > Jacqueline:
> > Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers, is recyclable and while some people have been using it for more than 20 years, others find it irritant.
> > René J.
> >
> > --- On Wed, 4/8/09, Jacqueline Farnsworth <Jacqueline.Farnsworth <@t> cls.ab.ca> wrote:
> >
> > From: Jacqueline Farnsworth <Jacqueline.Farnsworth <@t> cls.ab.ca>
> > Subject: [Histonet] Formula 83 users?
> > To: "Histonet" <Histonet <@t> lists.utsouthwestern.edu>
> > Date: Wednesday, April 8, 2009, 12:31 PM
> >
> > HI all.
> > I have searched the Histonet archives and found a lot of very positive reviews
> > regarding Formula 83. I am wondering if anyone has encountered any issues with
> > using Formula 83 on a stainer and then xylene on a tape coverslipper. Are there
> > any miscibility issues? Are you soaking in xylene prior to tape coverslipping
> > required? Any helpful hints/tips/tricks?
> >
> > Thank you in advance.
> >
> >
> > Jacqueline Farnsworth
> > Anatomic Pathology, Tech III
> > Foothills Medical Centre
> > Calgary Laboratory Services
> >
> > Ph: 403-944-1578
> > Fax: 403-944-4748
> > P Please consider the environment before printing this email.
> >
> > ________________________________
> > This message and any attached documents are only for the use of the intended
> > recipient(s), are confidential and may contain privileged information. Any
> > unauthorized review, use, retransmission, or other disclosure is strictly
> > prohibited. If you have received this message in error, please notify the sender
> > immediately, and then delete the original message. Thank you.
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 6
> > Date: Thu, 9 Apr 2009 06:58:45 -0700 (PDT)
> > From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> > Subject: Re: [Histonet] Negative IHC controls
> > To: histonet <@t> lists.utsouthwestern.edu, Sheila Haas
> > <micropathlabs <@t> yahoo.com>
> > Message-ID: <292901.13223.qm <@t> web65712.mail.ac4.yahoo.com>
> > Content-Type: text/plain; charset=iso-8859-1
> >
> > I used to run a negative control per case or per block within any given case when doing IHC, but not for reagents.
> > René J.
> >
> > --- On Wed, 4/8/09, Sheila Haas <micropathlabs <@t> yahoo.com> wrote:
> >
> > From: Sheila Haas <micropathlabs <@t> yahoo.com>
> > Subject: [Histonet] Negative IHC controls
> > To: histonet <@t> lists.utsouthwestern.edu
> > Date: Wednesday, April 8, 2009, 2:57 PM
> >
> > I have a question concerning ANP.22570 on the CAP checklist. Could you all
> > tell me how you are handling
> > negative controls for IHC staining? The question actually states (and I've
> > confirmed with CAP) that we should be running two types of negative controls.
> > One for reagents and one for each antibody in a run. I'd like to know
> > what the practice is. This seems very costly and time consuming. Thanks in
> > advance!
> >
> > Sheila Haas
> > Laboratory Supervisor
> > Micro Path Laboratories
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 7
> > Date: Thu, 9 Apr 2009 10:14:48 -0400
> > From: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>
> > Subject: RE: [Histonet] Formula 83 users?
> > To: "'rjbuesa <@t> yahoo.com'" <rjbuesa <@t> yahoo.com>, Histonet
> > <Histonet <@t> lists.utsouthwestern.edu>, Jacqueline Farnsworth
> > <Jacqueline.Farnsworth <@t> cls.ab.ca>
> > Message-ID:
> > <5A2BD13465E061429D6455C8D6B40E3908778D156E <@t> IBMB7Exchange.digestivespecialists.com>
> >
> > Content-Type: text/plain; charset="iso-8859-1"
> >
> >
> > With all due respect René; I have been using Formula 83 with my coverslipper with no problems at all though have a glass coverslipper not a tape.
> > Linda
> >
> >
> > Linda Blazek HT (ASCP)
> > Manager/Supervisor
> > GI Pathology of Dayton
> > 7415 Brandt Pike
> > Huber Heights, OH 45424
> > Phone: (937) 293-4424 ext 7118
> > Email: lblazek <@t> digestivespecialists.com
> >
> >
> >
> > -----Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
> > Sent: Thursday, April 09, 2009 9:56 AM
> > To: Histonet; Jacqueline Farnsworth
> > Subject: Re: [Histonet] Formula 83 users?
> >
> > Jacqueline:
> > Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers, is recyclable and while some people have been using it for more than 20 years, others find it irritant.
> > René J.
> >
> > --- On Wed, 4/8/09, Jacqueline Farnsworth <Jacqueline.Farnsworth <@t> cls.ab.ca> wrote:
> >
> > From: Jacqueline Farnsworth <Jacqueline.Farnsworth <@t> cls.ab.ca>
> > Subject: [Histonet] Formula 83 users?
> > To: "Histonet" <Histonet <@t> lists.utsouthwestern.edu>
> > Date: Wednesday, April 8, 2009, 12:31 PM
> >
> > HI all.
> > I have searched the Histonet archives and found a lot of very positive reviews
> > regarding Formula 83. I am wondering if anyone has encountered any issues with
> > using Formula 83 on a stainer and then xylene on a tape coverslipper. Are there
> > any miscibility issues? Are you soaking in xylene prior to tape coverslipping
> > required? Any helpful hints/tips/tricks?
> >
> > Thank you in advance.
> >
> >
> > Jacqueline Farnsworth
> > Anatomic Pathology, Tech III
> > Foothills Medical Centre
> > Calgary Laboratory Services
> >
> > Ph: 403-944-1578
> > Fax: 403-944-4748
> > P Please consider the environment before printing this email.
> >
> > ________________________________
> > This message and any attached documents are only for the use of the intended
> > recipient(s), are confidential and may contain privileged information. Any
> > unauthorized review, use, retransmission, or other disclosure is strictly
> > prohibited. If you have received this message in error, please notify the sender
> > immediately, and then delete the original message. Thank you.
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> > ------------------------------
> >
> > Message: 8
> > Date: Thu, 09 Apr 2009 10:11:11 -0400
> > From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
> > Subject: Re: [Histonet] Spyware from an Attachment:
> > To: u know <u_deserve_2_know <@t> yahoo.com>
> > Cc: histonet <@t> lists.utsouthwestern.edu
> > Message-ID: <49DE01FF.100 <@t> umdnj.edu>
> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed
> >
> > Dear List:
> >
> > I strongly suggest that you NOT use any links offered by people with no
> > name and no affiliation. Yes, there are good spyware detection programs
> > available at no cost (Adaware for example) but I don't think this is one
> > of them. There are at least 3 potential saboteurs on this list.
> >
> > Geoff
> >
> > u know wrote:
> >
> >> Dear Listers,
> >>
> >> If you ever opened any strange attachments from this list, you should really consider scanning your Hard Disk for spyware. There is a great free anti-spyware program we use, which you can get at http://tinyurl.com/ynupj4 <----that is a virus-proof link, by the way.
> >>
> >>
> >>
> >>
> >> _______________________________________________
> >> Histonet mailing list
> >> Histonet <@t> lists.utsouthwestern.edu
> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >>
> >>
> >>
> >>
> >
> >
> >
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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