[Histonet] Re: TMAs for Cell Pellets - Histonet Digest, Vol 65, Issue 22

Lori Charette lac65 <@t> email.med.yale.edu
Thu Apr 16 09:11:49 CDT 2009 

Cell pellet density is important and the medium in which the cells are 
suspended in (i.e. agar, etc...) makes a difference as well.
The medium consistency and type is important to the cell block 
preparation if they are to be used in a TMA.
This is true when cutting tissue micro-arrays that contain both cell 
pellets and tissue samples. If you are looking to make TMA's
of cell-pellets, tissue, or both on a regular basis then perhaps 
purchasing an arrayer is the way to go but for making control TMA's
or TMA's of interest then there are other avenues.
Lori


Thom Jensen wrote:
> If you are making a cell blocks with your cell pellets then punching 
> the cell block and inserting it into a TMA then is ideal.  The cell 
> pellets we have made were created like a cell block.  The cells are 
> spon down, the fluid is poured off and the cell button is mixed with a 
> augar type substance (venders would have info on the augar type 
> material used for making cell blocks).  Once the solution solidifies 
> it is placed in a paper or mesh bage and placed into a cassette for 
> processing.  Once processed the cells are imbedded into a paraffin 
> block as you would tissue.  A fresh H&E is cut off the block and areas 
> of interest are marked on the slide to show the best spot to punch for 
> the TMA. You will also find that the TMA instrument can pay for itself 
> sometimes after one protocal.  By this I mean you will save on 
> antibody cost and have consistency in your staining since all samples 
> are on one slide instead of many slides.  I have constructed regular 
> paraffin TMAs, cell arrays, sponge arrays (cells growing in a sponge 
> type material processed and embedded in paraffin) and frozen TMAs.  It 
> just takes a little expermenting, some patience and pratice making TMAs.
>
> cheers,
> Thom
>
>
> > Date: Tue, 14 Apr 2009 10:20:40 -0400
> > From: lac65 <@t> email.med.yale.edu
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: [Histonet] Re: Histonet Digest, Vol 65, Issue 22
> >
> > Give me a shout in regards to cell pellets & TMA's.
> > There are several methods and some are less expensive then others.
> > Have a good one.
> > Lori
> >
> >
> >
> > Beecher is usually difficult to deal
> > with.histonet-request <@t> lists.utsouthwestern.edu wrote:
> > > Send Histonet mailing list submissions to
> > > histonet <@t> lists.utsouthwestern.edu
> > >
> > > To subscribe or unsubscribe via the World Wide Web, visit
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > > or, via email, send a message with subject or body 'help' to
> > > histonet-request <@t> lists.utsouthwestern.edu
> > >
> > > You can reach the person managing the list at
> > > histonet-owner <@t> lists.utsouthwestern.edu
> > >
> > > When replying, please edit your Subject line so it is more specific
> > > than "Re: Contents of Histonet digest..."
> > >
> > >
> > > Today's Topics:
> > >
> > > 1. Re: TMA for cell pellets? (Bernie Taupin)
> > > 2. To all spam gourmets (V. Neubert)
> > > 3. RE: I recommend NOT opening that Attachment: (Hugh Luk)
> > > 4. RE: TMA for cell pellets? (Hartson, Louise)
> > > 5. Re: Formula 83 users? (Rene J Buesa)
> > > 6. Re: Negative IHC controls (Rene J Buesa)
> > > 7. RE: Formula 83 users? (Blazek, Linda)
> > > 8. Re: Spyware from an Attachment: (Geoff McAuliffe)
> > > 9. Formula 83-Thank you! (Jacqueline Farnsworth)
> > > 10. HISTOS 5 processor (FU,DONGTAO)
> > > 11. Position Open In Mass (Alyssa Peterson)
> > > 12. Re: HISTOS 5 processor (Robert Schoonhoven)
> > > 13. Travelling Histotechs (Robert Schoonhoven)
> > > 14. Regional Sales Manager position- West Coast
> > > (Kris.Caldwell <@t> leica-microsystems.com)
> > > 15. New lab, need some info (Putnam, Jodi)
> > >
> > >
> > > ----------------------------------------------------------------------
> > >
> > > Message: 1
> > > Date: Wed, 8 Apr 2009 22:50:26 -0700 (PDT)
> > > From: Bernie Taupin <bernietaupin <@t> ymail.com>
> > > Subject: Re: [Histonet] TMA for cell pellets?
> > > To: Thom Jensen <tissuearray <@t> hotmail.com>,
> > > louise_hartson <@t> urmc.rochester.edu, histonet <@t> lists.utsouthwestern.edu
> > > Message-ID: <338565.20208.qm <@t> web43513.mail.sp1.yahoo.com>
> > > Content-Type: text/plain; charset=iso-8859-1
> > >
> > > Recently, someone mentioned putting a library of protocols online, 
> perhaps in the HistoNet archives. I'd like to second that emotion if 
> its possible to make happen... What a great resource that would be!
> > >
> > >
> > >
> > >
> > > ________________________________
> > > From: Thom Jensen <tissuearray <@t> hotmail.com>
> > > To: louise_hartson <@t> urmc.rochester.edu; 
> histonet <@t> lists.utsouthwestern.edu
> > > Sent: Thursday, April 9, 2009 1:27:18 AM
> > > Subject: [Histonet] TMA for cell pellets?
> > >
> > >
> > >
> > > Have you tried to embed your cell pellets into a tissue 
> microarray? Two companies I would recommend:
> > > www.arraymold.com
> > > www.beecherinstruments.com
> > >
> > > Two of the best TMA products on the market.
> > >
> > >
> > > cheers,
> > >
> > >
> > >> Date: Thu, 2 Apr 2009 12:05:39 -0400
> > >> From: Louise_Hartson <@t> URMC.Rochester.edu
> > >> To: histonet <@t> lists.utsouthwestern.edu
> > >> Subject: [Histonet] Embedding cell pellets
> > >>
> > >>
> > >> I am looking for a protocol for embedding cell pellets in paraffin.
> > >> Thanks,
> > >> Louise
> > >>
> > >> Louise Hartson, BA
> > >> Senior Technical Associate
> > >> University of Rochester
> > >> Louise_Hartson <@t> URMC.Rochester.edu
> > >> _______________________________________________
> > >> Histonet mailing list
> > >> Histonet <@t> lists.utsouthwestern.edu
> > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > >>
> > >
> > > _________________________________________________________________
> > > Hotmail® is up to 70% faster. Now good news travels really fast.
> > > 
> http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009_______________________________________________
> > > Histonet mailing list
> > > Histonet <@t> lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > >
> > >
> > >
> > >
> > >
> > > ------------------------------
> > >
> > > Message: 2
> > > Date: Thu, 09 Apr 2009 10:07:54 +0200
> > > From: "V. Neubert" <histonet.nospam <@t> vneubert.com>
> > > Subject: [Histonet] To all spam gourmets
> > > To: histonet <@t> lists.utsouthwestern.edu
> > > Message-ID: <49DDACDA.8040503 <@t> vneubert.com>
> > > Content-Type: text/plain; charset=ISO-8859-1; format=flowed
> > >
> > > Please, stop spamming this list.
> > > Comment if you know something useful. Otherwise keep it.
> > > I don't mind what you had for breakfast in June 1st last year or your
> > > opinion on my clothes' colour.
> > >
> > > Believe it or not, there IS a real life ( link:
> > > http://en.wikipedia.org/wiki/Real_life_(reality) ), and maybe you 
> should
> > > check it out - it has way more to offer than waiting for responses to
> > > mock about.
> > >
> > > Thanks.
> > > Valentin
> > >
> > > PS: What about a moderated board? Every inappropriate comment on 
> HISTO
> > > TOPIC could be moved or even deleted, except in the spamming forum 
> which
> > > can be found on nearly every board on the net.
> > >
> > >
> > >
> > > ------------------------------
> > >
> > > Message: 3
> > > Date: Wed, 8 Apr 2009 22:13:22 -1000
> > > From: Hugh Luk <hlukey <@t> msn.com>
> > > Subject: RE: [Histonet] I recommend NOT opening that Attachment:
> > > To: histonet <histonet <@t> lists.utsouthwestern.edu>
> > > Message-ID: <SNT106-W30443621932D763B828123C3830 <@t> phx.gbl>
> > > Content-Type: text/plain; charset="iso-8859-1"
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > > Fellow histology and related fields professionals,
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > > I'm not opening this. From a call name of "u know 
> (u_deserve_2_know <@t> yahoo.com)." The last time I opened something like 
> this, from a name like this, I ended up owing money for Viagra 
> treatments for Panda bears or something like that. It may be real, but 
> it probably is spam. There...has been...a lot...of it...recently!
> > >
> > > By the way, there is no such thing as a "Virus-proof LINK!" Not 
> sure? Just Google it.
> > >
> > > If you need Spyware or Antivirus software, check your IT 
> department. Also, CNET has free downloads of AdAware, or AVG antivirus 8.
> > >
> > > And now for a joke (I think we all need it); A physician is doing 
> his rounds and a nurse stops him in the hallway, "Doctor, can you sign 
> these reports?" "Fine" he says and proceeds to pull out a rectal 
> thermometer from his coat pocket. He looks at it in disbelief and 
> exclaims, "Darn it! Some a$$ has my pen!"
> > >
> > > Respectfully,
> > > Hugh Luk, HTL (ASCP)
> > > Cancer Research center of Hawaii
> > > Pathology shared resources lab manager
> > > KP- Hawaii
> > >
> > >
> > >
> > >
> > >> Date: Wed, 8 Apr 2009 21:41:35 -0700
> > >> From: u_deserve_2_know <@t> yahoo.com
> > >> To: histonet <@t> lists.utsouthwestern.edu
> > >> Subject: [Histonet] Spyware from an Attachment:
> > >>
> > >> Dear Listers,
> > >>
> > >> If you ever opened any strange attachments from this list, you 
> should really consider scanning your Hard Disk for spyware. There is a 
> great free anti-spyware program we use, which you can get at 
> http://DELETED<----that is a virus-proof link, by the way.
> > >>
> > >>
> > >>
> > >>
> > >> _______________________________________________
> > >> Histonet mailing list
> > >> Histonet <@t> lists.utsouthwestern.edu
> > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > >>
> > >
> > > _________________________________________________________________
> > > Quick access to your favorite MSN content and Windows Live with 
> Internet Explorer 8.
> > > 
> http://ie8.msn.com/microsoft/internet-explorer-8/en-us/ie8.aspx?ocid=B037MSN55C0701A
> > >
> > > ------------------------------
> > >
> > > Message: 4
> > > Date: Thu, 9 Apr 2009 08:29:23 -0400
> > > From: "Hartson, Louise" <Louise_Hartson <@t> URMC.Rochester.edu>
> > > Subject: RE: [Histonet] TMA for cell pellets?
> > > To: "Bernie Taupin" <bernietaupin <@t> ymail.com>, "Thom Jensen"
> > > <tissuearray <@t> hotmail.com>, <histonet <@t> lists.utsouthwestern.edu>
> > > Message-ID:
> > > 
> <1089A756B010074CA09E65CD92401608018A8CAC <@t> MEDMAIL.urmc-sh.rochester.edu>
> > >
> > > Content-Type: text/plain; charset="iso-8859-1"
> > >
> > > Thank you to everyone who responded!! I was given the cell pellets 
> already in the matrix so I only had to process!! It was easier than 
> the mouse tissues!! Since we do so few 20-40 at a time I do everything 
> by hand...no automation in our lab!!
> > >
> > >
> > > -----Original Message-----
> > > From: Bernie Taupin [mailto:bernietaupin <@t> ymail.com]
> > > Sent: Thu 4/9/2009 1:50 AM
> > > To: Thom Jensen; Hartson, Louise; histonet <@t> lists.utsouthwestern.edu
> > > Subject: Re: [Histonet] TMA for cell pellets?
> > >
> > > Recently, someone mentioned putting a library of protocols online, 
> perhaps in the HistoNet archives. I'd like to second that emotion if 
> its possible to make happen... What a great resource that would be!
> > >
> > >
> > >
> > >
> > > ________________________________
> > > From: Thom Jensen <tissuearray <@t> hotmail.com>
> > > To: louise_hartson <@t> urmc.rochester.edu; 
> histonet <@t> lists.utsouthwestern.edu
> > > Sent: Thursday, April 9, 2009 1:27:18 AM
> > > Subject: [Histonet] TMA for cell pellets?
> > >
> > >
> > >
> > > Have you tried to embed your cell pellets into a tissue 
> microarray? Two companies I would recommend:
> > > www.arraymold.com
> > > www.beecherinstruments.com
> > >
> > > Two of the best TMA products on the market.
> > >
> > >
> > > cheers,
> > >
> > >
> > >> Date: Thu, 2 Apr 2009 12:05:39 -0400
> > >> From: Louise_Hartson <@t> URMC.Rochester.edu
> > >> To: histonet <@t> lists.utsouthwestern.edu
> > >> Subject: [Histonet] Embedding cell pellets
> > >>
> > >>
> > >> I am looking for a protocol for embedding cell pellets in paraffin.
> > >> Thanks,
> > >> Louise
> > >>
> > >> Louise Hartson, BA
> > >> Senior Technical Associate
> > >> University of Rochester
> > >> Louise_Hartson <@t> URMC.Rochester.edu
> > >> _______________________________________________
> > >> Histonet mailing list
> > >> Histonet <@t> lists.utsouthwestern.edu
> > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > >>
> > >
> > > _________________________________________________________________
> > > Hotmail® is up to 70% faster. Now good news travels really fast.
> > > 
> http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009_______________________________________________
> > > Histonet mailing list
> > > Histonet <@t> lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > > ------------------------------
> > >
> > > Message: 5
> > > Date: Thu, 9 Apr 2009 06:56:21 -0700 (PDT)
> > > From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> > > Subject: Re: [Histonet] Formula 83 users?
> > > To: Histonet <Histonet <@t> lists.utsouthwestern.edu>, Jacqueline
> > > Farnsworth <Jacqueline.Farnsworth <@t> cls.ab.ca>
> > > Message-ID: <613905.80912.qm <@t> web65715.mail.ac4.yahoo.com>
> > > Content-Type: text/plain; charset=iso-8859-1
> > >
> > > Jacqueline:
> > > Formula 83 is a naphthenic hydrocarbon that cannot be used with 
> coverslippers, is recyclable and while some people have been using it 
> for more than 20 years, others find it irritant.
> > > René J.
> > >
> > > --- On Wed, 4/8/09, Jacqueline Farnsworth 
> <Jacqueline.Farnsworth <@t> cls.ab.ca> wrote:
> > >
> > > From: Jacqueline Farnsworth <Jacqueline.Farnsworth <@t> cls.ab.ca>
> > > Subject: [Histonet] Formula 83 users?
> > > To: "Histonet" <Histonet <@t> lists.utsouthwestern.edu>
> > > Date: Wednesday, April 8, 2009, 12:31 PM
> > >
> > > HI all.
> > > I have searched the Histonet archives and found a lot of very 
> positive reviews
> > > regarding Formula 83. I am wondering if anyone has encountered any 
> issues with
> > > using Formula 83 on a stainer and then xylene on a tape 
> coverslipper. Are there
> > > any miscibility issues? Are you soaking in xylene prior to tape 
> coverslipping
> > > required? Any helpful hints/tips/tricks?
> > >
> > > Thank you in advance.
> > >
> > >
> > > Jacqueline Farnsworth
> > > Anatomic Pathology, Tech III
> > > Foothills Medical Centre
> > > Calgary Laboratory Services
> > >
> > > Ph: 403-944-1578
> > > Fax: 403-944-4748
> > > P Please consider the environment before printing this email.
> > >
> > > ________________________________
> > > This message and any attached documents are only for the use of 
> the intended
> > > recipient(s), are confidential and may contain privileged 
> information. Any
> > > unauthorized review, use, retransmission, or other disclosure is 
> strictly
> > > prohibited. If you have received this message in error, please 
> notify the sender
> > > immediately, and then delete the original message. Thank you.
> > > _______________________________________________
> > > Histonet mailing list
> > > Histonet <@t> lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > >
> > >
> > >
> > >
> > >
> > > ------------------------------
> > >
> > > Message: 6
> > > Date: Thu, 9 Apr 2009 06:58:45 -0700 (PDT)
> > > From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> > > Subject: Re: [Histonet] Negative IHC controls
> > > To: histonet <@t> lists.utsouthwestern.edu, Sheila Haas
> > > <micropathlabs <@t> yahoo.com>
> > > Message-ID: <292901.13223.qm <@t> web65712.mail.ac4.yahoo.com>
> > > Content-Type: text/plain; charset=iso-8859-1
> > >
> > > I used to run a negative control per case or per block within any 
> given case when doing IHC, but not for reagents.
> > > René J.
> > >
> > > --- On Wed, 4/8/09, Sheila Haas <micropathlabs <@t> yahoo.com> wrote:
> > >
> > > From: Sheila Haas <micropathlabs <@t> yahoo.com>
> > > Subject: [Histonet] Negative IHC controls
> > > To: histonet <@t> lists.utsouthwestern.edu
> > > Date: Wednesday, April 8, 2009, 2:57 PM
> > >
> > > I have a question concerning ANP.22570 on the CAP checklist. Could 
> you all
> > > tell me how you are handling
> > > negative controls for IHC staining? The question actually states 
> (and I've
> > > confirmed with CAP) that we should be running two types of 
> negative controls.
> > > One for reagents and one for each antibody in a run. I'd like to know
> > > what the practice is. This seems very costly and time consuming. 
> Thanks in
> > > advance!
> > >
> > > Sheila Haas
> > > Laboratory Supervisor
> > > Micro Path Laboratories
> > >
> > >
> > >
> > > _______________________________________________
> > > Histonet mailing list
> > > Histonet <@t> lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > >
> > >
> > >
> > >
> > >
> > > ------------------------------
> > >
> > > Message: 7
> > > Date: Thu, 9 Apr 2009 10:14:48 -0400
> > > From: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>
> > > Subject: RE: [Histonet] Formula 83 users?
> > > To: "'rjbuesa <@t> yahoo.com'" <rjbuesa <@t> yahoo.com>, Histonet
> > > <Histonet <@t> lists.utsouthwestern.edu>, Jacqueline Farnsworth
> > > <Jacqueline.Farnsworth <@t> cls.ab.ca>
> > > Message-ID:
> > > 
> <5A2BD13465E061429D6455C8D6B40E3908778D156E <@t> IBMB7Exchange.digestivespecialists.com>
> > >
> > > Content-Type: text/plain; charset="iso-8859-1"
> > >
> > >
> > > With all due respect René; I have been using Formula 83 with my 
> coverslipper with no problems at all though have a glass coverslipper 
> not a tape.
> > > Linda
> > >
> > >
> > > Linda Blazek HT (ASCP)
> > > Manager/Supervisor
> > > GI Pathology of Dayton
> > > 7415 Brandt Pike
> > > Huber Heights, OH 45424
> > > Phone: (937) 293-4424 ext 7118
> > > Email: lblazek <@t> digestivespecialists.com
> > >
> > >
> > >
> > > -----Original Message-----
> > > From: histonet-bounces <@t> lists.utsouthwestern.edu 
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J 
> Buesa
> > > Sent: Thursday, April 09, 2009 9:56 AM
> > > To: Histonet; Jacqueline Farnsworth
> > > Subject: Re: [Histonet] Formula 83 users?
> > >
> > > Jacqueline:
> > > Formula 83 is a naphthenic hydrocarbon that cannot be used with 
> coverslippers, is recyclable and while some people have been using it 
> for more than 20 years, others find it irritant.
> > > René J.
> > >
> > > --- On Wed, 4/8/09, Jacqueline Farnsworth 
> <Jacqueline.Farnsworth <@t> cls.ab.ca> wrote:
> > >
> > > From: Jacqueline Farnsworth <Jacqueline.Farnsworth <@t> cls.ab.ca>
> > > Subject: [Histonet] Formula 83 users?
> > > To: "Histonet" <Histonet <@t> lists.utsouthwestern.edu>
> > > Date: Wednesday, April 8, 2009, 12:31 PM
> > >
> > > HI all.
> > > I have searched the Histonet archives and found a lot of very 
> positive reviews
> > > regarding Formula 83. I am wondering if anyone has encountered any 
> issues with
> > > using Formula 83 on a stainer and then xylene on a tape 
> coverslipper. Are there
> > > any miscibility issues? Are you soaking in xylene prior to tape 
> coverslipping
> > > required? Any helpful hints/tips/tricks?
> > >
> > > Thank you in advance.
> > >
> > >
> > > Jacqueline Farnsworth
> > > Anatomic Pathology, Tech III
> > > Foothills Medical Centre
> > > Calgary Laboratory Services
> > >
> > > Ph: 403-944-1578
> > > Fax: 403-944-4748
> > > P Please consider the environment before printing this email.
> > >
> > > ________________________________
> > > This message and any attached documents are only for the use of 
> the intended
> > > recipient(s), are confidential and may contain privileged 
> information. Any
> > > unauthorized review, use, retransmission, or other disclosure is 
> strictly
> > > prohibited. If you have received this message in error, please 
> notify the sender
> > > immediately, and then delete the original message. Thank you.
> > > _______________________________________________
> > > Histonet mailing list
> > > Histonet <@t> lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > >
> > >
> > >
> > >
> > > _______________________________________________
> > > Histonet mailing list
> > > Histonet <@t> lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > >
> > >
> > >
> > > ------------------------------
> > >
> > > Message: 8
> > > Date: Thu, 09 Apr 2009 10:11:11 -0400
> > > From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
> > > Subject: Re: [Histonet] Spyware from an Attachment:
> > > To: u know <u_deserve_2_know <@t> yahoo.com>
> > > Cc: histonet <@t> lists.utsouthwestern.edu
> > > Message-ID: <49DE01FF.100 <@t> umdnj.edu>
> > > Content-Type: text/plain; charset=ISO-8859-1; format=flowed
> > >
> > > Dear List:
> > >
> > > I strongly suggest that you NOT use any links offered by people 
> with no
> > > name and no affiliation. Yes, there are good spyware detection 
> programs
> > > available at no cost (Adaware for example) but I don't think this 
> is one
> > > of them. There are at least 3 potential saboteurs on this list.
> > >
> > > Geoff
> > >
> > > u know wrote:
> > >
> > >> Dear Listers,
> > >>
> > >> If you ever opened any strange attachments from this list, you 
> should really consider scanning your Hard Disk for spyware. There is a 
> great free anti-spyware program we use, which you can get at 
> http://tinyurl.com/ynupj4 <----that is a virus-proof link, by the way.
> > >>
> > >>
> > >>
> > >>
> > >> _______________________________________________
> > >> Histonet mailing list
> > >> Histonet <@t> lists.utsouthwestern.edu
> > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > >>
> > >>
> > >>
> > >>
> > >
> > >
> > >
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------------------------------------------------
> Rediscover Hotmail®: Now available on your iPhone or BlackBerry Check 
> it out. 
> <http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Mobile1_042009>

More information about the Histonet mailing list