[Histonet] Re: Histonet Digest, Vol 65, Issue 22

Tue Apr 14 09:20:40 CDT 2009

Give me a shout in regards to cell pellets & TMA's.
There are several methods and some are less expensive then others.
Have a good one.
Lori

Beecher is usually difficult to deal 
with.histonet-request <@t> lists.utsouthwestern.edu wrote:
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> Today's Topics:
>
>    1. Re: TMA for cell pellets? (Bernie Taupin)
>    2. To all spam gourmets (V. Neubert)
>    3. RE: I recommend NOT opening that Attachment: (Hugh Luk)
>    4. RE: TMA for cell pellets? (Hartson, Louise)
>    5. Re: Formula 83 users? (Rene J Buesa)
>    6. Re: Negative IHC controls (Rene J Buesa)
>    7. RE: Formula 83 users? (Blazek, Linda)
>    8. Re: Spyware from an Attachment: (Geoff McAuliffe)
>    9. Formula 83-Thank you! (Jacqueline Farnsworth)
>   10. HISTOS 5 processor (FU,DONGTAO)
>   11. Position Open In Mass (Alyssa Peterson)
>   12. Re: HISTOS 5 processor (Robert Schoonhoven)
>   13. Travelling Histotechs (Robert Schoonhoven)
>   14. Regional Sales Manager position- West Coast
>       (Kris.Caldwell <@t> leica-microsystems.com)
>   15. New lab, need some info (Putnam, Jodi)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 8 Apr 2009 22:50:26 -0700 (PDT)
> From: Bernie Taupin <bernietaupin <@t> ymail.com>
> Subject: Re: [Histonet] TMA for cell pellets?
> To: Thom Jensen <tissuearray <@t> hotmail.com>,
> 	louise_hartson <@t> urmc.rochester.edu, 	histonet <@t> lists.utsouthwestern.edu
> Message-ID: <338565.20208.qm <@t> web43513.mail.sp1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Recently, someone mentioned putting a library of protocols online, perhaps in the HistoNet archives. I'd like to second that emotion if its possible to make happen... What a great resource that would be!
>
>
>
>
> ________________________________
> From: Thom Jensen <tissuearray <@t> hotmail.com>
> To: louise_hartson <@t> urmc.rochester.edu; histonet <@t> lists.utsouthwestern.edu
> Sent: Thursday, April 9, 2009 1:27:18 AM
> Subject: [Histonet] TMA for cell pellets?
>
>
>
> Have you tried to embed your cell pellets into a tissue microarray?  Two companies I would recommend:
> www.arraymold.com
> www.beecherinstruments.com
>
> Two of the best TMA products on the market.
>
>
> cheers,
>
>   
>> Date: Thu, 2 Apr 2009 12:05:39 -0400
>> From: Louise_Hartson <@t> URMC.Rochester.edu
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Embedding cell pellets
>>
>>
>> I am looking for a protocol for embedding cell pellets in paraffin.
>> Thanks,
>> Louise
>>
>> Louise Hartson, BA
>> Senior Technical Associate
>> University of Rochester
>> Louise_Hartson <@t> URMC.Rochester.edu
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>     
>
> _________________________________________________________________
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>
>
>       
>
> ------------------------------
>
> Message: 2
> Date: Thu, 09 Apr 2009 10:07:54 +0200
> From: "V. Neubert" <histonet.nospam <@t> vneubert.com>
> Subject: [Histonet] To all spam gourmets
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <49DDACDA.8040503 <@t> vneubert.com>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Please, stop spamming this list.
> Comment if you know something useful. Otherwise keep it.
> I don't mind what you had for breakfast in June 1st last year or your 
> opinion on my clothes' colour.
>
> Believe it or not, there IS a real life ( link: 
> http://en.wikipedia.org/wiki/Real_life_(reality) ), and maybe you should 
> check it out - it has way more to offer than waiting for responses to 
> mock about.
>
> Thanks.
> Valentin
>
> PS: What about a moderated board? Every inappropriate comment on HISTO 
> TOPIC could be moved or even deleted, except in the spamming forum which 
> can be found on nearly every board on the net.
>
>
>
> ------------------------------
>
> Message: 3
> Date: Wed, 8 Apr 2009 22:13:22 -1000
> From: Hugh Luk <hlukey <@t> msn.com>
> Subject: RE: [Histonet] I recommend NOT opening that Attachment:
> To: histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <SNT106-W30443621932D763B828123C3830 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
>
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>
> Fellow histology and related fields professionals,
>
>
>
>
>
>
>
>
>
>
>
> I'm not opening this.  From a call name of "u know (u_deserve_2_know <@t> yahoo.com)."  The last time I opened something like this, from a name like this, I ended up owing money for Viagra treatments for Panda bears or something like that.  It may be real, but it probably is spam.  There...has been...a lot...of it...recently!
>
> By the way, there is no such thing as a "Virus-proof LINK!"  Not sure?  Just Google it.
>
> If you need Spyware or Antivirus software, check your IT department.  Also, CNET has free downloads of AdAware, or AVG antivirus 8. 
>
> And now for a joke (I think we all need it); A physician is doing his rounds and a nurse stops him in the hallway, "Doctor, can you sign these reports?"  "Fine" he says and proceeds to pull out a rectal thermometer from his coat pocket.  He looks at it in disbelief and exclaims, "Darn it!  Some a$$ has my pen!"
>
> Respectfully,
> Hugh Luk, HTL (ASCP)
> Cancer Research center of Hawaii
> Pathology shared resources lab manager
> KP- Hawaii 
>
>
>
>   
>> Date: Wed, 8 Apr 2009 21:41:35 -0700
>> From: u_deserve_2_know <@t> yahoo.com
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Spyware from an Attachment:
>>
>> Dear Listers,
>>
>> If you ever opened any strange attachments from this list, you should really consider scanning your Hard Disk for spyware. There is a great free anti-spyware program we use, which you can get at http://DELETED<----that is a virus-proof link, by the way.
>>
>>
>>
>>       
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>     
>
> _________________________________________________________________
> Quick access to your favorite MSN content and Windows Live with Internet Explorer 8. 
> http://ie8.msn.com/microsoft/internet-explorer-8/en-us/ie8.aspx?ocid=B037MSN55C0701A
>
> ------------------------------
>
> Message: 4
> Date: Thu, 9 Apr 2009 08:29:23 -0400
> From: "Hartson, Louise" <Louise_Hartson <@t> URMC.Rochester.edu>
> Subject: RE: [Histonet] TMA for cell pellets?
> To: "Bernie Taupin" <bernietaupin <@t> ymail.com>,	"Thom Jensen"
> 	<tissuearray <@t> hotmail.com>,	<histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> 	<1089A756B010074CA09E65CD92401608018A8CAC <@t> MEDMAIL.urmc-sh.rochester.edu>
> 	
> Content-Type: text/plain;	charset="iso-8859-1"
>
> Thank you to everyone who responded!!  I was given the cell pellets already in the matrix so I only had to process!!  It was easier than the mouse tissues!!  Since we do so few 20-40 at a time I do everything by hand...no automation in our lab!!
>
>
> -----Original Message-----
> From: Bernie Taupin [mailto:bernietaupin <@t> ymail.com]
> Sent: Thu 4/9/2009 1:50 AM
> To: Thom Jensen; Hartson, Louise; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] TMA for cell pellets?
>  
> Recently, someone mentioned putting a library of protocols online, perhaps in the HistoNet archives. I'd like to second that emotion if its possible to make happen... What a great resource that would be!
>
>
>
>
> ________________________________
> From: Thom Jensen <tissuearray <@t> hotmail.com>
> To: louise_hartson <@t> urmc.rochester.edu; histonet <@t> lists.utsouthwestern.edu
> Sent: Thursday, April 9, 2009 1:27:18 AM
> Subject: [Histonet] TMA for cell pellets?
>
>
>
> Have you tried to embed your cell pellets into a tissue microarray?  Two companies I would recommend:
> www.arraymold.com
> www.beecherinstruments.com
>
> Two of the best TMA products on the market.
>
>
> cheers,
>
>   
>> Date: Thu, 2 Apr 2009 12:05:39 -0400
>> From: Louise_Hartson <@t> URMC.Rochester.edu
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Embedding cell pellets
>>
>>
>> I am looking for a protocol for embedding cell pellets in paraffin.
>> Thanks,
>> Louise
>>
>> Louise Hartson, BA
>> Senior Technical Associate
>> University of Rochester
>> Louise_Hartson <@t> URMC.Rochester.edu
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>     
>
> _________________________________________________________________
> Hotmail® is up to 70% faster. Now good news travels really fast.
> http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009_______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>       
>
>
>
> ------------------------------
>
> Message: 5
> Date: Thu, 9 Apr 2009 06:56:21 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Formula 83 users?
> To: Histonet <Histonet <@t> lists.utsouthwestern.edu>,	Jacqueline
> 	Farnsworth <Jacqueline.Farnsworth <@t> cls.ab.ca>
> Message-ID: <613905.80912.qm <@t> web65715.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Jacqueline:
> Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers, is recyclable and while some people have been using it for more than 20 years, others find it irritant.
> René J.
>
> --- On Wed, 4/8/09, Jacqueline Farnsworth <Jacqueline.Farnsworth <@t> cls.ab.ca> wrote:
>
> From: Jacqueline Farnsworth <Jacqueline.Farnsworth <@t> cls.ab.ca>
> Subject: [Histonet] Formula 83 users?
> To: "Histonet" <Histonet <@t> lists.utsouthwestern.edu>
> Date: Wednesday, April 8, 2009, 12:31 PM
>
> HI all.
> I have searched the Histonet archives and found a lot of very positive reviews
> regarding Formula 83. I am wondering if anyone has encountered any issues with
> using Formula 83 on a stainer and then xylene on a tape coverslipper.  Are there
> any miscibility issues? Are you soaking in xylene prior to tape coverslipping
> required? Any helpful hints/tips/tricks?
>
> Thank you in advance.
>
>
> Jacqueline Farnsworth
> Anatomic Pathology, Tech III
> Foothills Medical Centre
> Calgary Laboratory Services
>
> Ph: 403-944-1578
> Fax: 403-944-4748
> P Please consider the environment before printing this email.
>
> ________________________________
> This message and any attached documents are only for the use of the intended
> recipient(s), are confidential and may contain privileged information. Any
> unauthorized review, use, retransmission, or other disclosure is strictly
> prohibited. If you have received this message in error, please notify the sender
> immediately, and then delete the original message. Thank you.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>       
>
> ------------------------------
>
> Message: 6
> Date: Thu, 9 Apr 2009 06:58:45 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Negative IHC controls
> To: histonet <@t> lists.utsouthwestern.edu, Sheila Haas
> 	<micropathlabs <@t> yahoo.com>
> Message-ID: <292901.13223.qm <@t> web65712.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> I used to run a negative control per case or per block within any given case when doing IHC, but not for reagents.
> René J.
>
> --- On Wed, 4/8/09, Sheila Haas <micropathlabs <@t> yahoo.com> wrote:
>
> From: Sheila Haas <micropathlabs <@t> yahoo.com>
> Subject: [Histonet] Negative IHC controls
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Wednesday, April 8, 2009, 2:57 PM
>
> I have a question concerning ANP.22570 on the CAP checklist. Could you all
> tell me how you are handling
> negative controls for IHC staining? The question actually states (and I've
> confirmed with CAP) that we should be running two types of negative controls.
> One for reagents and one for each antibody in a run. I'd like to know
> what the practice is. This seems very costly and time consuming. Thanks in
> advance!
>  
> Sheila Haas
> Laboratory Supervisor
> Micro Path Laboratories
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>       
>
> ------------------------------
>
> Message: 7
> Date: Thu, 9 Apr 2009 10:14:48 -0400
> From: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>
> Subject: RE: [Histonet] Formula 83 users?
> To: "'rjbuesa <@t> yahoo.com'" <rjbuesa <@t> yahoo.com>, Histonet
> 	<Histonet <@t> lists.utsouthwestern.edu>, Jacqueline Farnsworth
> 	<Jacqueline.Farnsworth <@t> cls.ab.ca>
> Message-ID:
> 	<5A2BD13465E061429D6455C8D6B40E3908778D156E <@t> IBMB7Exchange.digestivespecialists.com>
> 	
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> With all due respect René; I have been using Formula 83 with my coverslipper with no problems at all though have a glass coverslipper not a tape.
> Linda 
>
>
> Linda Blazek HT (ASCP)
> Manager/Supervisor
> GI Pathology of Dayton
> 7415 Brandt Pike
> Huber Heights, OH 45424
> Phone: (937) 293-4424 ext 7118
> Email: lblazek <@t> digestivespecialists.com
>
>  
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
> Sent: Thursday, April 09, 2009 9:56 AM
> To: Histonet; Jacqueline Farnsworth
> Subject: Re: [Histonet] Formula 83 users?
>
> Jacqueline:
> Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers, is recyclable and while some people have been using it for more than 20 years, others find it irritant.
> René J.
>
> --- On Wed, 4/8/09, Jacqueline Farnsworth <Jacqueline.Farnsworth <@t> cls.ab.ca> wrote:
>
> From: Jacqueline Farnsworth <Jacqueline.Farnsworth <@t> cls.ab.ca>
> Subject: [Histonet] Formula 83 users?
> To: "Histonet" <Histonet <@t> lists.utsouthwestern.edu>
> Date: Wednesday, April 8, 2009, 12:31 PM
>
> HI all.
> I have searched the Histonet archives and found a lot of very positive reviews
> regarding Formula 83. I am wondering if anyone has encountered any issues with
> using Formula 83 on a stainer and then xylene on a tape coverslipper.  Are there
> any miscibility issues? Are you soaking in xylene prior to tape coverslipping
> required? Any helpful hints/tips/tricks?
>
> Thank you in advance.
>
>
> Jacqueline Farnsworth
> Anatomic Pathology, Tech III
> Foothills Medical Centre
> Calgary Laboratory Services
>
> Ph: 403-944-1578
> Fax: 403-944-4748
> P Please consider the environment before printing this email.
>
> ________________________________
> This message and any attached documents are only for the use of the intended
> recipient(s), are confidential and may contain privileged information. Any
> unauthorized review, use, retransmission, or other disclosure is strictly
> prohibited. If you have received this message in error, please notify the sender
> immediately, and then delete the original message. Thank you.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>       
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 8
> Date: Thu, 09 Apr 2009 10:11:11 -0400
> From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
> Subject: Re: [Histonet] Spyware from an Attachment:
> To: u know <u_deserve_2_know <@t> yahoo.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <49DE01FF.100 <@t> umdnj.edu>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Dear List:
>
> I strongly suggest that you NOT use any links offered by people with no 
> name and no affiliation. Yes, there are good spyware detection programs 
> available at no cost (Adaware for example) but I don't think this is one 
> of them. There are at least 3 potential saboteurs on this list.
>
> Geoff
>
> u know wrote:
>   
>> Dear Listers,
>>
>> If you ever opened any strange attachments from this list, you should really consider scanning your Hard Disk for spyware. There is a great free anti-spyware program we use, which you can get at http://tinyurl.com/ynupj4 <----that is a virus-proof link, by the way.
>>
>>
>>
>>       
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>   
>>     
>
>
>   