[Histonet] decaling bones

Tue Apr 14 16:36:12 CDT 2009

Hazel
Processing using alcohol then xylene?
If so then suspect too long in alcohol or using xylene is your major problem with paraffin wax a close second.
Can use chloroform as an intermediary agent and bone will not harden as much.
If you must use xylene, try to soak in a mixture of xylene:paraffin wax (1:1) at room temperature for at least a few hours. This allows some wax to penetrate into the tissue and cuts down time in paraffin wax.
Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Tuesday, April 14, 2009 4:17 PM
To: rjbuesa <@t> yahoo.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] decaling bones

The bone appears soft and easy to cut with a razor blade but after processing the tissue is as hard as brick.   Any suggestions?   

Hazel Horn

Hazel Horn, HT/HTL (ASCP)

Supervisor of Histology

Arkansas Children's Hospital

1 Children's Way    Slot 820

Little Rock, AR   72202

phone   501.364.4240

fax        501.364.3155

visit us on the web at:    www.archildrens.org

________________________________

From: Rene J Buesa [mailto:rjbuesa <@t> yahoo.com] 
Sent: Tuesday, April 14, 2009 4:02 PM
To: histonet <@t> lists.utsouthwestern.edu; Horn, Hazel V
Subject: Re: [Histonet] decaling bones

No, once it is decalcified, that's it!
René J.

--- On Tue, 4/14/09, Horn, Hazel V <HornHV <@t> archildrens.org> wrote:

From: Horn, Hazel V <HornHV <@t> archildrens.org>
Subject: [Histonet] decaling bones
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, April 14, 2009, 12:04 PM

We are having the hardest time decaling bones.   Femurs and tibias.    

Is there such as thing as overdecaling and the bone becoming hard again?

Hazel Horn

Hazel Horn, HT/HTL (ASCP)

Supervisor of Histology

Arkansas Children's Hospital

1 Children's Way    Slot 820

Little Rock, AR   72202

phone   501.364.4240

fax        501.364.3155

visit us on the web at:    www.archildrens.org

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