[Histonet] decaling bones

[Horn, Hazel V]

The bone appears soft and easy to cut with a razor blade but after processing the tissue is as hard as brick.   Any suggestions?   

 

Hazel Horn

Hazel Horn, HT/HTL (ASCP)

Supervisor of Histology

Arkansas Children's Hospital

1 Children's Way    Slot 820

Little Rock, AR   72202

 

phone   501.364.4240

fax        501.364.3155

 

visit us on the web at:    www.archildrens.org

 

________________________________

From: Rene J Buesa [mailto:rjbuesa <@t> yahoo.com] 
Sent: Tuesday, April 14, 2009 4:02 PM
To: histonet <@t> lists.utsouthwestern.edu; Horn, Hazel V
Subject: Re: [Histonet] decaling bones

 

No, once it is decalcified, that's it!
René J.

--- On Tue, 4/14/09, Horn, Hazel V <HornHV <@t> archildrens.org> wrote:

From: Horn, Hazel V <HornHV <@t> archildrens.org>
Subject: [Histonet] decaling bones
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, April 14, 2009, 12:04 PM

We are having the hardest time decaling bones.   Femurs and tibias.    
 
 
 
Is there such as thing as overdecaling and the bone becoming hard again?
 
 
 
Hazel Horn
 
Hazel Horn, HT/HTL (ASCP)
 
Supervisor of Histology
 
Arkansas Children's Hospital
 
1 Children's Way    Slot 820
 
Little Rock, AR   72202
 
 
 
phone   501.364.4240
 
fax        501.364.3155
 
 
 
visit us on the web at:    www.archildrens.org
 
 
 
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