[Histonet] Blocking

[Ian Montgomery]

            About to use the antibody anti-connexin 43. On the data sheet it
tells me to block using 5% milk, so it's casein blocking. In 1992 Dave Tacha
published a paper (J. Histotech. 15. 132-137. Casein reduces non-specific
background staining in immunolabelling techniques), regarding casein
blocking where he recommended using it at 5%. Later however, Mary Vaughan on
Histonet suggested reducing the concentration, after a series of
experiments, to 0.03% for 30 minutes. 

            What's the current view on casein blocking, concentration, time
etc. 

Ian.  

 

Dr. Ian Montgomery,

Histotechnology,

I.B.L.S. Support Unit,

Thomson Building,

University of Glasgow,

Glasgow,

G12 8QQ.