[Histonet] processing artifact

[Lynette Pavelich]

Gloria,
We have had issues with our small biopsies in the past also.  We
actually had several things going on that we were able to solve with a
little help from our friends in the field!

Rene's explaination is very valid as it was also one of our issues. 
Since your processing schedule/rotation sounds good, have you considered
the type of cassettes you are using?  We were using Histoscreen
Cassettes and found that they processed good some days, and bad other
times........and this was with multiple biopsies in the same cassette. 
We found that during the processing, air bubbles within the cassettes
were causing the incomplete processing.  Those little screens, while
very handy in containing the specimen, created air bubbles when the
solutions were pumped in/out.  If one of the small biopsies got caught
in a air bubble, it missed that part of the processing.  That took us a
while to figure that one out.  Now that we've omitted them, our
inconsistant processing is solved.  We had little raisins for nuclei. 
Not good!!
Hope this helped.  Let us know what you find out......

Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503

ph:  810-257-9948
fax:  810-762-7082
>>> "Okland, Gloria" <gokland <@t> ameripath.com> 04/13/09 11:00 AM >>>
We are seeing this opaque artifact in some tissues.  It can affect one
biopsy but not the two others in the same cassette.  It is very random,
appears as this white haze, obscuring nuclear detail, making the slides
very difficult to read.  Thinking it was water in the processor, I did a
test run over the weekend and everything looks great.  No reagents had
been changed or adjustments made for the test run.  Any suggestions of
what else this could be and a remedy would be greatly appreciated!!..

Thank you.

Gloria


Gloria Okland
AmeriPath | Histology

Please think about resource conservation before you print this message

CONFIDENTIALITY NOTICE: This e-mail message, including any attachments,
is for the sole use of the intended recipient(s) and may contain
confidential and privileged information. Any unauthorized review, use,
disclosure or distribution is prohibited. If you are not the intended
recipient, please contact the sender by reply e-mail and destroy all
copies of the original message.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet