[Histonet] Edge effect on IHC-P

[Jan Shivers]

Sam,

Could you give us a few facts from your protocol?  They may help pin down 
what might be causing your edge effect.

How long does your tissue stay on the grossing/trimming table before being 
put into fixative?
What type of fixative are you using?  How fresh is it?
How long to you fix?
What temps to you perform IHC at?
How long are your incubations?
Etc.

Jan Shivers
UMN VDL

----- Original Message ----- 
From: "Perry, Samuel" <Samuel_Perry <@t> DFCI.HARVARD.EDU>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, April 09, 2009 1:31 PM
Subject: [Histonet] Edge effect on IHC-P


Hi All,

I am having variable staining when performing IHC.  I seem to get an edge 
effect
where the edge of the tissue shows increased DAB staining compared to the 
middle
of the tissue.  I have tried testing many fixation variables and still 
haven't
found an edge affect culprit.  I'm wondering if anyone in the community has
experienced and corrected this problem.  Thanks for you help!

-Sam Perry



Dana Farber Cancer Institute

Boston MA

Research Technician



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