[Histonet] Edge effect IHC-P follow-up

[Perry, Samuel]

I am seeing this when I work with mouse tissue.  After necropsy I extract the
tissue from the mouse, cassette it, put it in fresh 10% NBF and let the tissue
fix 24hrs before processing.  I also see an edge effect when I fix with 4% PFA.
During IHC-P I usually retrieve with a pressure cooker (120C) and citrate buffer
solution.  I use the DAKO envision kit and incubate the primary antibody at room
temp. for 1 hour.

 

I also see an edge affect when I take frozen tissue and thaw it in RT 10%NBF
overnight followed by processing.



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