[Histonet] Edge effect on IHC-P

[Perry, Samuel]

Hi All,

I am having variable staining when performing IHC.  I seem to get an edge effect
where the edge of the tissue shows increased DAB staining compared to the middle
of the tissue.  I have tried testing many fixation variables and still haven't
found an edge affect culprit.  I'm wondering if anyone in the community has
experienced and corrected this problem.  Thanks for you help!

-Sam Perry

 

Dana Farber Cancer Institute

Boston MA

Research Technician 



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