[Histonet] AE1/AE3 nuclear staining

[Mark Tarango]

When was the last time you checked the pH of your retrieval solution?  Or
are you using an enzyme?

Mark Tarango

On Tue, Apr 7, 2009 at 9:53 AM, Tammy Barnhart
<Tammy.Barnhart <@t> mckennan.org>wrote:

> We have recently been having dark nuclear staining with our AE1/AE3
> antibody.  This is a recent event and we cannot figure out what is going
> on.  It started gradually and has been increasing in intensity.  We have
> not changed antibody lots or any other part of our protocol.  Has anyone
> seen this before?  Any suggestions on what is happening here?
>
> Tammy Barnhart, BS, HTL(ASCP)
>
> Avera McKennan Hospital
>
>
>
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