[Histonet] AE1/AE3 nuclear staining

[Rene J Buesa]

Have you changed any of the reagents in your X-press tissue processor or other aspect of your tissue processing protocol? This could be a factor.
René J.

--- On Tue, 4/7/09, Tammy Barnhart <Tammy.Barnhart <@t> mckennan.org> wrote:

From: Tammy Barnhart <Tammy.Barnhart <@t> mckennan.org>
Subject: [Histonet] AE1/AE3 nuclear staining
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, April 7, 2009, 12:53 PM

We have recently been having dark nuclear staining with our AE1/AE3
antibody.  This is a recent event and we cannot figure out what is going
on.  It started gradually and has been increasing in intensity.  We have
not changed antibody lots or any other part of our protocol.  Has anyone
seen this before?  Any suggestions on what is happening here?  

Tammy Barnhart, BS, HTL(ASCP)

Avera McKennan Hospital



-----------------------------------------
Confidentiality Notice: This e-mail message, including any attachments, is for
the sole use of the intended recipient(s) and may contain confidential and
privileged information. Any unauthorized review, use, disclosure, or
distribution is prohibited. If you are not the intended recipient, please
contact the sender by reply e-mail and destroy all copies of the original
message.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet