[Histonet] AE1/AE3 nuclear staining

[Tony Henwood]

Possibly the concentration of hydrogen peroxide in the DAB solution has
been slowly increasing (maybe micropipette creep?)

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tammy
Barnhart
Sent: Wednesday, 8 April 2009 2:54 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] AE1/AE3 nuclear staining


We have recently been having dark nuclear staining with our AE1/AE3
antibody.  This is a recent event and we cannot figure out what is going
on.  It started gradually and has been increasing in intensity.  We have
not changed antibody lots or any other part of our protocol.  Has anyone
seen this before?  Any suggestions on what is happening here?  

Tammy Barnhart, BS, HTL(ASCP)

Avera McKennan Hospital



-----------------------------------------
Confidentiality Notice: This e-mail message, including any attachments,
is for the sole use of the intended recipient(s) and may contain
confidential and privileged information. Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are not the intended
recipient, please contact the sender by reply e-mail and destroy all
copies of the original message.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*********************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
**********************************************************************