[Histonet] AE1/AE3 nuclear staining

[Tammy Barnhart]

We have recently been having dark nuclear staining with our AE1/AE3
antibody.  This is a recent event and we cannot figure out what is going
on.  It started gradually and has been increasing in intensity.  We have
not changed antibody lots or any other part of our protocol.  Has anyone
seen this before?  Any suggestions on what is happening here?  

Tammy Barnhart, BS, HTL(ASCP)

Avera McKennan Hospital



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