[Histonet] "FREEZY" spray

[Ingles Claire]

Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut fatty sections in the cryostat without it!
Claire

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Federal registry 1910.1030
"All procedures involving blood or other potential infectious materials
shall be performed in such a manner as to minimize splashing, spraying,
spattering, or generation of droplets of these substances."

NCCLS Document M29-T (now CLSI)
"Frozen sections done on unfixed tissue pose a high risk because accidents
are common.  Freezing of tissue does not inactivate infectious agents.
Freezing propellants under pressure should not be used for frozen sections
as they may cause spattering of droplets of infectious material."

Based on the above statements, freezing spray should NOT be used in a
cryostat with unfixed tissue.

Jennifer MacDonald