[Histonet] questions about permiabilisation and fixation of nematode larva for further immunohistochemistry

[Ana Resendes]

Hello to the Histotects community worldwide.

Would like to know if someone out there has any suggestion or have worked
with nematode larva:

1- fixation and after  (protocol)
2-permeabilization of the chitin outer membrane    (protocol)

in order to:

3- adhere them to slides (maybe polylisine slides??? any other suggestion or
protocol for slide adhesion of larva)

4- Run a immunohistochemistry on them (suggestions or protocol for any
adjustments needed for further permiabilization and antigen retrieval if
needed)

Thanks
Ana

-- 
Ana Resendes
DVM, MSc, PhD
Veterinary Pathologist
Centro de Investigação de Recursos Naturais (CIRN)
Secção de Anatomia e Taxonomia Zoológicas, Departamento de Biologia,
Universidade dos Açores.
Rua da Mãe de Deus, 58 - Apartado 1422
P - 9501-801 Ponta Delgada (Açores)
Portugal
Tel. (+351)  296 650 000 ext. 1109
Fax  (+351) 296 650 100
http://www.uac.pt/~pherg/