[Histonet] Technovit 7200 (isobornylmethacrylate) staining

[Jack Ratliff]

To my knowledge it is near impossible to obtain an adequate H&E stain  
on thick resin sections. If this is not true, I would be interested in  
the same information.

Jack


On Apr 3, 2009, at 6:12 PM, "Garcia, Lori, Sr. Scientist" <lori.garcia <@t> medtronic.com 
 > wrote:

> This question is specifically for Linda Jenkins or anyone who has  
> worked
> with Technovit 7200,
>
> I am trying to do an H&E stain on ~70 um thick ground sections on
> plastic slides. I have followed the Donath method all the way through
> processing, but the sections do not pick up stains the way the booklet
> says they should. I am even having trouble following GMA staining
> procedures that I have found, in fact am afraid to try too many  
> because
> the acrylic forms tiny cracks that interfere with microscopy. I have
> researched the histonet archives, but haven't found anything other  
> than
> a couple of similar inquiries.
>
> I would very much appreciate any advice from people who have worked  
> with
> this specific formulation.
>
> Happy Friday!
>
> Lori
>
> [CONFIDENTIALITY AND PRIVACY NOTICE]
>
> Information transmitted by this email is proprietary to Medtronic  
> and is intended for use only by the individual or entity to which it  
> is addressed, and may contain information that is private,  
> privileged, confidential or exempt from disclosure under applicable  
> law. If you are not the intended recipient or it appears that this  
> mail has been forwarded to you without proper authority, you are  
> notified that any use or dissemination of this information in any  
> manner is strictly prohibited. In such cases, please delete this  
> mail from your records.
>
> To view this notice in other languages you can either select the  
> following link or manually copy and paste the link into the address  
> bar of a web browser: http://emaildisclaimer.medtronic.com
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>