[Histonet] Removing PMMA blocks from glass vials

[Pamela Marcum]

I agree.  It is a little messy and can be dangerous if you are not paying enough attention.  LN just gets the glass cold and cracks it so you don't need a hammer.  If you leave it in the LN too long the block may crack with the vial. 



Pam Marcum 

UPENN Vet School 

New Bolton Center 



----- Original Message ----- 
From: "Shakun Aswani" <Shakun.Aswani <@t> acologix.com> 
To: "Paul Monfils" <PMonfils <@t> Lifespan.org>, histonet <@t> lists.utsouthwestern.edu 
Sent: Friday, April 3, 2009 2:23:30 PM GMT -05:00 US/Canada Eastern 
Subject: RE: [Histonet] Removing PMMA blocks from glass vials 

Hi Paul, 
I did so many of those PMMA blocks cracking. Take a under pad, line your 
blocks not too many and cover with another under pad and start 
(literally) hammering them one by one with hammer gently I am not 
kidding. Make sure you wear safety glasses and double gloves to protect 
your hands. When the blocks are cracked remove them and rinse with tap 
water and small glass pieces will go into sharp container. I know seems 
very complicated but it is not. 
If you have more questions regarding this please fell free to contact 
me. 
Good Luck 
Shakun 

-----Original Message----- 
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Monfils, 
Paul 
Sent: Friday, April 03, 2009 11:00 AM 
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] Removing PMMA blocks from glass vials 

Someone gave me some small PMMA blocks polymerized inside glass 
scintillation vials.  I always use plastic vials.  Obviously the vials 
have to be broken to get the blocks out.  Does anyone have some advice 
on the best way to do this?  The guy who brought them said he thought I 
could "use liquid nitrogen", but had no specifics.  Any help will be 
appreciated. 
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