[Histonet] Burned Tissue

[Jennifer MacDonald]

We had this problem in the past, but it was attributed to the excessive 
use of freezing spray.  The tissue was basically freezer burnt.

Jennifer




thisisann <@t> aol.com 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
04/02/2009 11:35 AM

To
histonet <@t> lists.utsouthwestern.edu
cc

Subject
[Histonet] Burned Tissue






Recently we are experiencing random blocks of tissue (approx. 3 blocks out 
of 400/2x) that appear to be "burned".? The tissue is prostate and the 
staining looks dark.? Upon recutting, sometimes it is better, sometimes it 
is not, which negates my ability to rule out the stainer completely.

We process on a 2 hour schedule using the Peloris and all tissue is 
processed together.? I am not understanding how, out of a 12 part 
prostate, only one block stains so dark so randomly.? When recut, some 
blocks are better, some still have the dark staining.

Does anyone have any suggestions?

Ann
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet