[Histonet] Burned Tissue

[Tony Henwood]

I would suggest checking the fixation. We had similar problems if the
tissue was not completely fixed. 
Are the tissues free to move around in the cassettes (ie have free
unhindered access to the fixative)?

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
thisisann <@t> aol.com
Sent: Friday, 3 April 2009 5:33 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Burned Tissue


Recently we are experiencing random blocks of tissue (approx. 3 blocks
out of 400/2x) that appear to be "burned".? The tissue is prostate and
the staining looks dark.? Upon recutting, sometimes it is better,
sometimes it is not, which negates my ability to rule out the stainer
completely.

We process on a 2 hour schedule using the Peloris and all tissue is
processed together.? I am not understanding how, out of a 12 part
prostate, only one block stains so dark so randomly.? When recut, some
blocks are better, some still have the dark staining.

Does anyone have any suggestions?

Ann
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