[Histonet] AZF fixed bone marrows and IHC's

[Mark Tarango]

I had the same problem but our cores were fixed in 10% NBF.  I tried
everything I could think of to get it to work as far as retrieval, so I
ended up having to do a real TUNEL assay.  We had to assume the antibody
wasn't specific enough.  Same antibody and staining platform as you.

Mark

On Fri, Sep 12, 2008 at 12:24 PM, Christine Jacobs <jacobc <@t> mmc.org> wrote:

> Is anyone having problems with IHC staining after a combination os AZF
> fixation and decal? There was a recent string on the Histonet regarding
> B-Plus but I can't remember exactly what was discussed. We are experiencing
> problems with TdT staining. We get nothing on the core biopsies and only
> edge artifact staining on most clots. The FFPE thymus tissue looks GREAT. We
> are using Cell Marque's predilute antibody on the Ventana BenchMark XT.
> Any and all comments will be appreciated.
> Chris Jacobs, HT(ASCP), QIHC
> Jason Wheaton, HTL (ASCP)
> NorDx Labs
> Scarborough, ME
>
> CONFIDENTIALITY NOTICE:  This email message, including any attachments, is
> for the use of the intended recipient(s) only and may contain information
> that is privileged, confidential, and prohibited from unauthorized
> disclosure under applicable law.  If you are not the intended recipient of
> this message, any dissemination, distribution, or copying of this message is
> strictly prohibited.  If you received this message in error, please notify
> the sender by reply email and destroy all copies of the original message and
> attachments.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>