[Histonet] AZF fixed bone marrows and IHC's

[Mark Tarango]

Actually, i think the conclusion we reached was that the decal was the
problem...

On Fri, Sep 12, 2008 at 12:53 PM, Mark Tarango <marktarango <@t> gmail.com>wrote:

>  I had the same problem but our cores were fixed in 10% NBF.  I tried
> everything I could think of to get it to work as far as retrieval, so I
> ended up having to do a real TUNEL assay.  We had to assume the antibody
> wasn't specific enough.  Same antibody and staining platform as you.
>
> Mark
>
>   On Fri, Sep 12, 2008 at 12:24 PM, Christine Jacobs <jacobc <@t> mmc.org>wrote:
>
>> Is anyone having problems with IHC staining after a combination os AZF
>> fixation and decal? There was a recent string on the Histonet regarding
>> B-Plus but I can't remember exactly what was discussed. We are experiencing
>> problems with TdT staining. We get nothing on the core biopsies and only
>> edge artifact staining on most clots. The FFPE thymus tissue looks GREAT. We
>> are using Cell Marque's predilute antibody on the Ventana BenchMark XT.
>> Any and all comments will be appreciated.
>> Chris Jacobs, HT(ASCP), QIHC
>> Jason Wheaton, HTL (ASCP)
>> NorDx Labs
>> Scarborough, ME
>>
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>
>