[Histonet] Pig endothelium

[Alistair Lindsay]

Dear All,

Does anyone have any experience with staining pig endothelium, specifically
keeping rings of blood vessels alive?

I am collecting fresh tissue from the slaughterhouse and incubating it with
tumour necrosis factor to induce VCAM-1 expression.  However, I suspect that
the tissue quickly loses viability and this is why I can¹t visualise any
VCAM with IHC. 

Does anyone have any tips for keeping the rings of tissue
going/fixing/antigen retrieval?

Many thanks

Alistair Lindsay MBChB MRCP

Tel.: 07989404808

Alistair.Lindsay <@t> btinternet.com
Alistair.Lindsay <@t> cardiov.ox.ac.uk