[Histonet] Pig endothelium

[Weems, Joyce]

Lipstick, maybe? Sorry, couldn't help it... j

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Alistair Lindsay
Sent: Thursday, September 11, 2008 2:43 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Pig endothelium

Dear All,

Does anyone have any experience with staining pig endothelium, specifically keeping rings of blood vessels alive?

I am collecting fresh tissue from the slaughterhouse and incubating it with tumour necrosis factor to induce VCAM-1 expression.  However, I suspect that the tissue quickly loses viability and this is why I can¹t visualise any VCAM with IHC. 

Does anyone have any tips for keeping the rings of tissue going/fixing/antigen retrieval?

Many thanks

Alistair Lindsay MBChB MRCP

Tel.: 07989404808

Alistair.Lindsay <@t> btinternet.com
Alistair.Lindsay <@t> cardiov.ox.ac.uk


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