[Histonet] Unstained slide storage

[Rene J Buesa]

Decreased antigenicity applies to any section stored and unprotected from oxygen (air) oxidation.
René J.

--- On Thu, 9/11/08, MVaughan4 <@t> ucok.edu <MVaughan4 <@t> ucok.edu> wrote:

From: MVaughan4 <@t> ucok.edu <MVaughan4 <@t> ucok.edu>
Subject: [Histonet] Unstained slide storage
To: histonet <@t> lists.utsouthwestern.edu
Date: Thursday, September 11, 2008, 2:27 PM

Daniel,
I have found that saving slides with FFPE skin sections 5-10 micrometer 
thick, the antigenicity declines with age, even when stored in the fridge. 
I have tested slides from the same block freshly cut versus 2-3 years 
stored on a microscope slide in the fridge. Whether this applies to needle 
biopsies of tumor tissue I don't know...now I just section what I need and 
return the block to the fridge.
Mel


Melville B. Vaughan, Ph. D.
Assistant Professor of Biology
Campus Coordinator, NSF Sure-Step program
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034
phone 405-974-5725

Message: 15
Date: Thu, 11 Sep 2008 10:40:21 -0500
From: "Daniel Schneider" <dlschneider <@t> gmail.com>
Subject: [Histonet] Protocol: Saving unstained slides...
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
 <1085e7000809110840k4b370fc5ld3b7e5be3f738d36 <@t> mail.gmail.com>
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I thought I'd poll the histonet community on an issue we're facing:

On needle biopsies for tumor, what, if any, is your protocol
for making unstained slides "on the front end" for potential use with
immunostains later (so that the specimen isn't exhausted producing levels 
x
3, and you have good sections of tumor for immunostains)?

Is this protocol followed on all needle biopsies for tumor?

And what happens to any unused, unstained slides?  Are they discarded (if
so, after how long?) or kept indefinitely?

Thanks for your input!
Daniel Schneider


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