[Histonet] Differentiating a neutrophil from a de-granulated eosinophil ....antibody or stain?

[jstaruk]

Hi all,

Does anyone know if the anti-eosinophil antibody will adhere to a
degranulated eosinophil or does the antibody only stick to the eosinophil
granules and not the cell itself?

I'm performing a differential count in a certain fluid and I'm highly
suspicious that many of the neutrophils are actually de-granulated
eosinophils (a bit larger, thicker, less segmented nuclei, etc).  Is there
any other method to differentiate a neutrophils from a degranulated
eosinophil that I'm not aware of?

Thank you!

Jim

_____________________
     Jim Staruk
Mass Histology Service
www.masshistology.com