[Histonet] Differentiating a neutrophil from a de-granulated eosinophil ....antibody or stain?

[Mark Tarango]

Got a picture?

On 9/2/08, jstaruk <jstaruk <@t> masshistology.com> wrote:
> Hi all,
>
> Does anyone know if the anti-eosinophil antibody will adhere to a
> degranulated eosinophil or does the antibody only stick to the eosinophil
> granules and not the cell itself?
>
> I'm performing a differential count in a certain fluid and I'm highly
> suspicious that many of the neutrophils are actually de-granulated
> eosinophils (a bit larger, thicker, less segmented nuclei, etc).  Is there
> any other method to differentiate a neutrophils from a degranulated
> eosinophil that I'm not aware of?
>
> Thank you!
>
> Jim
>
> _____________________
>     Jim Staruk
> Mass Histology Service
> www.masshistology.com
>
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