[Histonet] RE: Dako and Leica immunostainers

[Sally Price]

All,
After reading this thread I just had offer my comments.  I'm not a big fan
of systems that do the dewax and AR, primarily because it costs way too much
to automate these steps.  I've never used the Bond, but I hear that you've
gotta put some plastic thingy - that probably costs too much - on top of
each slide, you gotta use their detection reagents - which probably
cost more than other companies, they charge you for empty barcoded
reagent containers, all the slides in the same tray have to use the same
detection reagents - which means that the continuous-feed feature has some
serious limits, it can't do double-stains, and they have less than 50
IVD-approved antbodies.  Can someone verify for me if all this issues are
true?  If so, why would someone want one of these stainers?  The Dako
stainer is a dinosaur and with all the newre/better ones available,
they should probably take it off the market.
Cheers,
Sally

-----Original Message-----

From: Josie Britton <JCBRITTON <@t> Cheshire-Med.COM>
To: Michael Bradley <jaustin1967 <@t> gmail.com>;
Histonet <@t> lists.utsouthwestern.edu
Sent: Tue, 18 Nov 2008 11:31 am
Subject: RE: [Histonet] Dako

Hi,
We just got the new Bond Max IHC stainer and we love it.  You just cut the
slides dry them and place them on the Bond.  It does retrieval, antibody
staining, and counter stain.  You just dehydrate , clear, and mount your
coverslip.  It is easy to use.  It has 3 individual slide tray's of 10.  You
can load more slides on the empty tray's and start a new batch while the
others are running.  We run into the pathologist's adding more antibodies to
the list an hour after we have run the first batch frequently, so this
feature is great.  When you add more IHC's the run time on all the slide
tray's run times do increase, but it's better than having to wait another
2-3 hours to put your next set of immuno's on.
Hope this helps!
Josie Britton HT
Cheshire Medical Center
580 Court Street
Keene, NH 03431

-----Original Message-----

Message: 9
Date: Wed, 19 Nov 2008 17:17:52 -0500
From: godsgalnow <@t> aol.com
Subject: Re: [Histonet] Dako
To: JCBRITTON <@t> Cheshire-Med.COM, jaustin1967 <@t> gmail.com,
    Histonet <@t> lists.utsouthwestern.edu

Just another FYI...
The Biocare IntelliPath does the same thing, but has 4 trays of 10 and it
allows you to make one of the trays?RUSH.? SO depending, on the volume?of
IHC you do either of these machines would work out great.
Roxanne