[Histonet] Another "Pen Shell" muscle issue

[Bryan Llewellyn]

Try using the picro-mallory.  This method differs from the usual trichromes 
by adding a step with small molecular weight, yellow acid dyes.  Possibly, 
the muscle in this mollusc may stain with them.

Technical details are at: 
http://stainsfile.info/StainsFile/stain/fibrin/picro-mallory-2.htm
For a discussion on how trichrome stains work go to: 
http://stainsfile.info/StainsFile/theory/tri_gen.htm

Bryan Llewellyn


----- Original Message ----- 
From: "Carlos Hotmail" <alzcarlos <@t> hotmail.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, November 13, 2008 7:24 PM
Subject: [Histonet] Another "Pen Shell" muscle issue


Hi all

I am returning to the histonet for some help.

The previous time I asked for help because I cannot stain correctly the 
muscle tissue of a mollusc, the "Pen Shell" Atrina maura.

I returned because neither PTAH, Mallory´s, Masson´s or Milligan´s 
trichromes stained consitently the muscle (smooth or striated) of this 
evasive species.
All the times I run a stain made some positives with sections of muscle from 
other organisms, vetebrate and invertebrates. And in all lf them the muscle 
looked right. All but the Pen Shell.

All the times almost all or every muscle cell stained as connective tissue, 
with very very small sections with a positive muscle reaction.

Anyone could help me to know how the tipical mammal muscle tissue stains?  I 
think if I know how the chemistry works I could find the exact problem with 
my stains.

All the samples are fixed in formalin 10% or Davidson, processed in ethanol 
series, Hemo-de (xilol substitute of limonene), paraplas plus and sectioned 
at 4 microns with a leica microtome.

thanks

(please give me an apologyze for my bad english writing)

Carlos Augusto Aguilar Cruz
Marine Biologist

Histology Laboratory at Unidad Pichilingue
Universidad Autónoma de Baja California Sur
La Paz, Baja California Sur, México
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