[Histonet] re: Biocare Decloaker HIER

[Linda M Watson]

Is the 118 psi a typo? I don't believe the instrument can go that 
high-max psi is 30.

Piero Nelva wrote:

> I used it for 4 minutes at about 118 psi and most antibodies worked 
> really well.  I was having huge problems with the Er and Her2 and this 
> solved them (almost) overnight.
>
>
> ----- Original Message ----- From: "Jan Shivers" <shive003 <@t> umn.edu>
> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
> Sent: Wednesday, November 05, 2008 3:12 AM
> Subject: [Histonet] re: Biocare Decloaker HIER
>
>
> For those of you who use Biocare's Decloaker for HIER on regular cases 
> (not prion protein retrieval), could you tell me how long you do HIER 
> with these machines?  I normally do my prion cases in a Decloaker for 
> 20', with a 25' cooldown, but I suspect that regular tissues don't 
> need that length of time at high pressure/temp.  I have an extra 
> Decloaker and am considering switching to this device for my normal 
> case work HIER, if it's time efficient.
>
> Please reply privately.   Thanks.
>
> Jan Shivers
> Senior Scientist
> Histology/IHC/EM Section Head
> Pathology Teaching Program
> University of Minnesota
> Veterinary Diagnostic Laboratory
> 1333 Gortner Ave.
> St. Paul, MN  55108
> 612-624-7297
> shive003 <@t> umn.edu
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