[Histonet] re: Biocare Decloaker HIER

[Piero Nelva]

Ooops.  One too many ones!

Piero

----- Original Message ----- 
From: "Linda M Watson" <Linda.Watson <@t> bms.com>
To: "Piero Nelva" <pieronelva01 <@t> bigpond.com>
Cc: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, November 05, 2008 11:07 PM
Subject: Re: [Histonet] re: Biocare Decloaker HIER


> Is the 118 psi a typo? I don't believe the instrument can go that
> high-max psi is 30.
>
> Piero Nelva wrote:
>
>> I used it for 4 minutes at about 118 psi and most antibodies worked
>> really well.  I was having huge problems with the Er and Her2 and this
>> solved them (almost) overnight.
>>
>>
>> ----- Original Message ----- From: "Jan Shivers" <shive003 <@t> umn.edu>
>> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
>> Sent: Wednesday, November 05, 2008 3:12 AM
>> Subject: [Histonet] re: Biocare Decloaker HIER
>>
>>
>> For those of you who use Biocare's Decloaker for HIER on regular cases
>> (not prion protein retrieval), could you tell me how long you do HIER
>> with these machines?  I normally do my prion cases in a Decloaker for
>> 20', with a 25' cooldown, but I suspect that regular tissues don't
>> need that length of time at high pressure/temp.  I have an extra
>> Decloaker and am considering switching to this device for my normal
>> case work HIER, if it's time efficient.
>>
>> Please reply privately.   Thanks.
>>
>> Jan Shivers
>> Senior Scientist
>> Histology/IHC/EM Section Head
>> Pathology Teaching Program
>> University of Minnesota
>> Veterinary Diagnostic Laboratory
>> 1333 Gortner Ave.
>> St. Paul, MN  55108
>> 612-624-7297
>> shive003 <@t> umn.edu
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>>
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>


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