[Histonet] re: Biocare Decloaker HIER

[Piero Nelva]

I used it for 4 minutes at about 118 psi and most antibodies worked really 
well.  I was having huge problems with the Er and Her2 and this solved them 
(almost) overnight.


----- Original Message ----- 
From: "Jan Shivers" <shive003 <@t> umn.edu>
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, November 05, 2008 3:12 AM
Subject: [Histonet] re: Biocare Decloaker HIER


For those of you who use Biocare's Decloaker for HIER on regular cases (not 
prion protein retrieval), could you tell me how long you do HIER with these 
machines?  I normally do my prion cases in a Decloaker for 20', with a 25' 
cooldown, but I suspect that regular tissues don't need that length of time 
at high pressure/temp.  I have an extra Decloaker and am considering 
switching to this device for my normal case work HIER, if it's time 
efficient.

Please reply privately.   Thanks.

Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu
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