AW: [Histonet] Kidney Bx question

[Gudrun Lang]

Would you be so kind and explain, why you have to do a short run. Are these
indeed NTX-biopsies, that need a report as fast as possible?
Or is it just the fear to over-process the biopsies?

We have ntx from time to time, but even then, the clinicians wait for the
report until the next day, noon. So we can process the biopsies with our
regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG =
kind of trichrome), immunohistochemistry and immunofluorescence. 
We process the needlebiopsies in sponges and with the regular 13-hour
protocol. Regarding IHC a fixation-time under 6 hours could also drive to
problems.

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
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Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von
histoinfo <@t> comcast.net
Gesendet: Donnerstag, 20. März 2008 14:01
An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist;
histonet <@t> lists.utsouthwestern.edu
Betreff: RE: [Histonet] Kidney Bx question


Rene,
Yes they are agitated. And the tearing problem that I originally described
is fairly infrequent. It's just that when it does happen we can't figure out
why. When it does happen nothing that we can see is any different than any
other day. Nothing in common as far as collection site either. I am
beginning to think more and more that it may be on days that we have 5 or 6
bxs that are all run together. But even then it is not every bx. I think we
will take better steps to prevent solution carryover and see what happens.
Of course it is so infrequent that we have this problem that it will be
months before we can say that we "fixed" the problem. I would still like to
compare times with others out there though. So please share your times for
hand processing.
Thanks,
Jennifer



-------------- Original message -------------- 
From: Rene J Buesa <rjbuesa <@t> yahoo.com> 

For hand processing at room temperature that schedule is too short, unless
the samples are agitated.
René J.

"Marshall Terry Dr, Consultant Histopathologist"
<Terry.Marshall <@t> rothgen.nhs.uk> wrote:
Do you have a train to catch?

Terry 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histoinfo <@t> comcast.net
Sent: 19 March 2008 17:14
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Kidney Bx question

We have been having some trouble with the inner surface of the tubules
looking torn look to the tissue. We hand process all our kidney bx's
with the times as follows.

1-formalin 15 minutes
2-50% ethanol 15 minutes
3-70% ethanol 15 minutes
4-80% ethanol 15 minutes
5-95% ethanol 15 minutes
6-100% ethanol 15 minutes
7-100% ethanol 15 minutes
9-xylene 15 minutes
10-xylene 15 minutes
Parraffin x2, 20 minutes each. 
They are then cut at 1 micron for PAS & JONES and 2 microns for H&E &
TRICHROME. 

We are trying to determine if this tearing is an artifact from
processing, collection or some other factor we haven't thought of. Has
anyone else come across this or a similar problem. All thoughts and
suggestions welcome.

Jennifer Saunders HT (ASCP)
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