[Histonet] Kidney Bx question

[histoinfo <@t> comcast.net]

Rene,
Yes they are agitated. And the tearing problem that I originally described is fairly infrequent. It's just that when it does happen we can't figure out why. When it does happen nothing that we can see is any different than any other day. Nothing in common as far as collection site either. I am beginning to think more and more that it may be on days that we have 5 or 6 bxs that are all run together. But even then it is not every bx. I think we will take better steps to prevent solution carryover and see what happens. Of course it is so infrequent that we have this problem that it will be months before we can say that we "fixed" the problem. I would still like to compare times with others out there though. So please share your times for hand processing.
Thanks,
Jennifer



-------------- Original message -------------- 
From: Rene J Buesa <rjbuesa <@t> yahoo.com> 

For hand processing at room temperature that schedule is too short, unless the samples are agitated.
René J.

"Marshall Terry Dr, Consultant Histopathologist" <Terry.Marshall <@t> rothgen.nhs.uk> wrote:
Do you have a train to catch?

Terry 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histoinfo <@t> comcast.net
Sent: 19 March 2008 17:14
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Kidney Bx question

We have been having some trouble with the inner surface of the tubules
looking torn look to the tissue. We hand process all our kidney bx's
with the times as follows.

1-formalin 15 minutes
2-50% ethanol 15 minutes
3-70% ethanol 15 minutes
4-80% ethanol 15 minutes
5-95% ethanol 15 minutes
6-100% ethanol 15 minutes
7-100% ethanol 15 minutes
9-xylene 15 minutes
10-xylene 15 minutes
Parraffin x2, 20 minutes each. 
They are then cut at 1 micron for PAS & JONES and 2 microns for H&E &
TRICHROME. 

We are trying to determine if this tearing is an artifact from
processing, collection or some other factor we haven't thought of. Has
anyone else come across this or a similar problem. All thoughts and
suggestions welcome.

Jennifer Saunders HT (ASCP)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
######################################################################
This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated.
The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed.
If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation.
######################################################################

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now.