[Histonet] C. Elegans fixation

[Danielle Crippen]

Hello Histo-experts,

 

Can you please share your favorite worm fixation methods with me??  I've
had great success in EM prep using 2% PFA 0.2% gluteraldehyde in
cocodylate buffer.  BUT-I had to "nick" the worms to get infiltration of
the fixative.  I now have a user who would prefer to avoid the "nicking"
technique for his application, but so far, we're experiencing
insufficient fixation.  Any/all advice is very welcome!!!

 

Many thanks in advance!

 

danielle

 

Danielle Crippen

Morphology and Imaging Core